Rabbit RIMBP2 Polyclonal Antibody | anti-RIMBP2 antibody

Anti-RIMBP2 Antibody Picoband

Cat. Num. AAA19999

• Gene Names: RIMBP2; RBP2; RIM-BP2; PPP1R133
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: RIMBP2, Antibody; Anti-RIMBP2 Antibody Picoband; Transmembrane protein 240; TMEM240; C1orf70; anti-RIMBP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-RIMBP2 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 0.25-0.5ug/ml, Human, Mouse, Rat; IHC(Paraffin-embedded Section): 2-5ug/ml, Mouse, Rat; IF: 5ug/ml, Mouse, Rat; FC/FACS (Fixed): 1-3ug /1x10⁶ cells, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human RIMBP2 recombinant protein (Position: K351-H1049).
• Subcellular Localization: Cell junction, synapse, Cell membrane, Multi-pass membrane protein
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins.
• Protein Function: Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 6. Flow Cytometry analysis of U20S cells using anti-RIMBP2 antibody (AAA19999).Overlay histogram showing U20S cells stained with AAA19999 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIMBP2 Antibody (AAA19999, 1ug/1x106 cells) for 30 min at 20 degree C. PE conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence)

(Figure 5. IF analysis of RIMBP2 using anti-RIMBP2 antibody (AAA19999).RIMBP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RIMBP2 Antibody (AAA19999) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence)

(Figure 4. IF analysis of RIMBP2 using anti-RIMBP2 antibody (AAA19999).RIMBP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RIMBP2 Antibody (AAA19999) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of RIMBP2 using anti-RIMBP2 antibody (AAA19999).RIMBP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RIMBP2 Antibody (AAA19999) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RIMBP2 Antibody (AAA19999) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tisue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMBP2 antigen affinity purified polyclonal antibody (#AAA19999) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RIMBP2 at approximately 150 kDa. The expected band size for RIMBP2 is at 116 kDa.)

Related Product Information for anti-RIMBP2 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-RIMBP2 antibody

Primary Antibodies; Cell Biology

References

1. Mittelstaedt, T., Schoch, S. Structure and evolution of RIM-BP genes: identification of a novel family member. Gene 403: 70-79, 2007.2. Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997.3. Wang, Y., Sugita, S., Sudhof, T. C. The RIM/NIM family of neuronal C2 domain proteins: interactions with Rab3 and a new class of Src homology 3 domain proteins. J. Biol. Chem. 275: 20033-20044, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 140561070
• NCBI GeneID: 23504
• NCBI Accession #: NP_056162.4
• NCBI GenBank Nucleotide #: NM_015347.4
• UniProt Accession #: O15034; Q96ID2
• Molecular Weight: 116,026 Da
• NCBI Official Full Name: RIMS-binding protein 2
• NCBI Official Synonym Full Names: RIMS binding protein 2
• NCBI Official Symbol: RIMBP2
• NCBI Official Synonym Symbols: RBP2; RIM-BP2; PPP1R133
• NCBI Protein Information: RIMS-binding protein 2; RIM binding protein 2; protein phosphatase 1, regulatory subunit 133
• UniProt Protein Name: RIMS-binding protein 2
• UniProt Gene Name: RIMBP2
• UniProt Synonym Gene Names: KIAA0318; RBP2; RIM-BP2
• UniProt Entry Name: RIMB2_HUMAN