WB (Western Blot)
(Western blot analysis of RIOK1 using HeLa whole cell lysates)
Rabbit RIOK1 Polyclonal Antibody | anti-RIOK1 antibody
RIOK1 Antibody
Gene Names
RIOK1; RIO1; AD034; RRP10; bA288G3.1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purified
Synonyms
RIOK1, Antibody; RIOK1 Antibody; AD034; bA288G3.1; RIO kinase 1 (yeast); RIO kinase 1; RIOK1; RIOK1_HUMAN; Serine/threonine-protein kinase rio1; anti-RIOK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RIOK1 antibody detects endogenous levels of total RIOK1
Purity/Purification
Peptide affinity purified
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
568
Applicable Applications for anti-RIOK1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human RIOK1, corresponding to a region within the C-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of RIOK1 using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of RIOK1 using HeLa whole cell lysates)
IHC (Immunohistochemistry)
(AAA328160 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA328160 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-RIOK1 antibody
Function: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503).
Subunit Structure: Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus) (PubMed:22072790, PubMed:21081503). Interacts with WDR77 (PubMed:22072790). Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation (PubMed:21081503).
Similarity: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
Subunit Structure: Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus) (PubMed:22072790, PubMed:21081503). Interacts with WDR77 (PubMed:22072790). Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation (PubMed:21081503).
Similarity: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66kDa
NCBI Official Full Name
serine/threonine-protein kinase RIO1 isoform 1
NCBI Official Synonym Full Names
RIO kinase 1
NCBI Official Symbol
RIOK1
NCBI Official Synonym Symbols
RIO1; AD034; RRP10; bA288G3.1
NCBI Protein Information
serine/threonine-protein kinase RIO1
UniProt Protein Name
Serine/threonine-protein kinase RIO1
UniProt Gene Name
RIOK1
Similar Products
Product Notes
The RIOK1 riok1 (Catalog #AAA328160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RIOK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RIOK1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RIOK1 riok1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIOK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.