WB (Western Blot)
(Detection of human RLIP76 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RLIP76 antibody AAA212728 (lot AAA212728-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human RLIP76 Polyclonal Antibody | anti-RLIP76 antibody
Rabbit anti-RLIP76 Antibody, Affinity Purified
Gene Names
RALBP1; RIP1; RLIP1; RLIP76
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RLIP76, Antibody; Rabbit anti-RLIP76 Antibody, Affinity Purified; RalBP1; RLIP76; RLIP1; ral-interacting protein 1; ralA-binding protein 1; DNP-SG ATPase; dinitrophenyl S-glutathione ATPase; 76 kDa Ral-interacting protein; RIP1; RalA-binding protein 1; ralA binding protein 1; anti-RLIP76 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RLIP76 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 605 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RLIP76 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RLIP76 antibody AAA212728 (lot AAA212728-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human RLIP76 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RLIP76 antibody AAA212728 (lot AAA212728-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RLIP76 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RLIP76 antibody AAA212728 (lot AAA212728-1) used for IP at 6 ug per reaction. RLIP76 was also immunoprecipitated by rabbit anti-RLIP76 antibody For blotting immunoprecipitated RLIP76, AAA212728 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RLIP76 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RLIP76 antibody AAA212728 (lot AAA212728-1) used for IP at 6 ug per reaction. RLIP76 was also immunoprecipitated by rabbit anti-RLIP76 antibody For blotting immunoprecipitated RLIP76, AAA212728 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-RLIP76 antibody
76 kDa Ral-interacting protein (RLIP76) can activate, specifically, hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. RLIP76 mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. It can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. It serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15311].
Product Categories/Family for anti-RLIP76 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76063 MW
NCBI Official Full Name
ralA-binding protein 1
NCBI Official Synonym Full Names
ralA binding protein 1
NCBI Official Symbol
RALBP1
NCBI Official Synonym Symbols
RIP1; RLIP1; RLIP76
NCBI Protein Information
ralA-binding protein 1
UniProt Protein Name
RalA-binding protein 1
UniProt Gene Name
RALBP1
UniProt Synonym Gene Names
RLIP1; RLIP76; RalBP1; DNP-SG ATPase
UniProt Entry Name
RBP1_HUMAN
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Product Notes
The RLIP76 ralbp1 (Catalog #AAA212728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RLIP76 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RLIP76 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RLIP76 ralbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RLIP76, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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