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product-image-AAA230350_WB10.jpg WB (Western Blot) (Western BlotPositive WB detected in: MCF-7 whole cell lysate,Hela whole cell lysate,HL-60 whole cell lysateAll lanes: RNF133 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43 KDaObserved band size: 43 KDa)

Rabbit anti-Human RNF133 Polyclonal Antibody | anti-RNF133 antibody

RNF133 Antibody

Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
RNF133, Antibody; RNF133 Antibody; E3 ubiquitin-protein ligase RNF133 ; RING finger protein 133; RNF133; anti-RNF133 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
376
Applicable Applications for anti-RNF133 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human E3 ubiquitin-protein ligase RNF133 protrin
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-163302
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

WB (Western Blot)

(Western BlotPositive WB detected in: MCF-7 whole cell lysate,Hela whole cell lysate,HL-60 whole cell lysateAll lanes: RNF133 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43 KDaObserved band size: 43 KDa)

product-image-AAA230350_WB10.jpg WB (Western Blot) (Western BlotPositive WB detected in: MCF-7 whole cell lysate,Hela whole cell lysate,HL-60 whole cell lysateAll lanes: RNF133 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43 KDaObserved band size: 43 KDa)

IHC (Immunohistochemisry)

(Immunofluorescent analysis of Hela cells using AAA230350 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

product-image-AAA230350_IHC11.jpg IHC (Immunohistochemisry) (Immunofluorescent analysis of Hela cells using AAA230350 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human testis using AAA230350 at dilution 1:100)

product-image-AAA230350_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human testis using AAA230350 at dilution 1:100)

WB (Western Blot)

(Western blotAll lanes: RNF133 antibody at 2ug/mlLane 1:K562 whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 42kDaObserved band size: 42kDa)

product-image-AAA230350_WB15.jpg WB (Western Blot) (Western blotAll lanes: RNF133 antibody at 2ug/mlLane 1:K562 whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 42kDaObserved band size: 42kDa)
Related Product Information for anti-RNF133 antibody
Has E3 ubiquitin-protein ligase activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,294 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF133
NCBI Official Synonym Full Names
ring finger protein 133
NCBI Official Symbol
RNF133
NCBI Protein Information
E3 ubiquitin-protein ligase RNF133
UniProt Protein Name
E3 ubiquitin-protein ligase RNF133
UniProt Gene Name
RNF133
UniProt Entry Name
RN133_HUMAN

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Product Notes

The RNF133 rnf133 (Catalog #AAA230350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF133 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF133 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RNF133 rnf133 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF133, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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