WB (Western Blot)
(Anti-RNF135 Antibody (C-term) at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human RNF135 Polyclonal Antibody | anti-RNF135 antibody
RNF135 Antibody (C-term)
Gene Names
RNF135; L13; MMFD; REUL; Riplet
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF135, Antibody; RNF135 Antibody (C-term); E3 ubiquitin-protein ligase RNF135; 632-; RIG-I E3 ubiquitin ligase; REUL; RING finger protein 135; Riplet; RNF135; anti-RNF135 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RNF135 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-401 amino acids from the C-terminal region of human RNF135.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
375-401
Sequence Length
286
Applicable Applications for anti-RNF135 antibody
ELISA, WB (Western Blot)
Function
E2-dependent E3 ubiquitin-protein ligase that functions as a RIG-I/DDX58 coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response (PubMed: , PubMed:19484123, PubMed: , PubMed:23950712, PubMed: , PubMed:31006531). Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway (PubMed: , PubMed:21147464, PubMed: , PubMed:31006531). Through a ubiquitin-independent parallelmechanism, which consists in bridging RIG-I/DDX58 filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway (PubMed: ). This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway (PubMed: , PubMed:31006531).
Cellular Location
Cytoplasm. Cytoplasm, Stress granule
Tissue Location
Expressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung. functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is located in a chromosomal region known to be frequently deleted in patients with neurofibromatosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-RNF135 Antibody (C-term) at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-RNF135 Antibody (C-term) at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(RNF135 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF135 antibody detected the RNF135 protein (arrow).)
WB (Western Blot)
(RNF135 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF135 antibody detected the RNF135 protein (arrow).)
Related Product Information for anti-RNF135 antibody
The protein encoded by this gene contains a RING finger
domain, a motif present in a variety of functionally distinct
proteins and known to be involved in protein-protein and
protein-DNA interactions. This gene is located in a chromosomal
region known to be frequently deleted in patients with
neurofibromatosis. Alternatively spliced transcript variants
encoding distinct isoforms have been reported. [provided by
RefSeq].
domain, a motif present in a variety of functionally distinct
proteins and known to be involved in protein-protein and
protein-DNA interactions. This gene is located in a chromosomal
region known to be frequently deleted in patients with
neurofibromatosis. Alternatively spliced transcript variants
encoding distinct isoforms have been reported. [provided by
RefSeq].
Product Categories/Family for anti-RNF135 antibody
References
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
You, F., et al. Nat. Immunol. 10(12):1300-1308(2009)
Visser, R., et al. Am. J. Med. Genet. A 149A (4), 806-808 (2009) :
Oshiumi, H., et al. J. Biol. Chem. 284(2):807-817(2009)
Gao, D., et al. PLoS ONE 4 (6), E5760 (2009) :
You, F., et al. Nat. Immunol. 10(12):1300-1308(2009)
Visser, R., et al. Am. J. Med. Genet. A 149A (4), 806-808 (2009) :
Oshiumi, H., et al. J. Biol. Chem. 284(2):807-817(2009)
Gao, D., et al. PLoS ONE 4 (6), E5760 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47888
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF135 isoform 3
NCBI Official Synonym Full Names
ring finger protein 135
NCBI Official Symbol
RNF135
NCBI Official Synonym Symbols
L13; MMFD; REUL; Riplet
NCBI Protein Information
E3 ubiquitin-protein ligase RNF135
UniProt Protein Name
E3 ubiquitin-protein ligase RNF135
UniProt Gene Name
RNF135
UniProt Synonym Gene Names
REUL
UniProt Entry Name
RN135_HUMAN
Similar Products
Product Notes
The RNF135 rnf135 (Catalog #AAA284366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 375-401. The RNF135 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF135 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RNF135 rnf135 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF135, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.