IHC (Immunohistochemisry)
(AAA323136 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit RNF156 Polyclonal Antibody | anti-RNF156 antibody
RNF156 Antibody
Gene Names
MGRN1; RNF156
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RNF156, Antibody; RNF156 Antibody; E3 ubiquitin-protein ligase MGRN1; KIAA0544; Mahogunin ring finger 1; Mahogunin RING finger protein 1; MGRN 1; Mgrn1; MGRN1_HUMAN; Probable E3 ubiquitin protein ligase MGRN1; RING finger protein 156; RNF 156; anti-RNF156 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RNF156 antibody detects endogenous levels of RNF156.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
554
Applicable Applications for anti-RNF156 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human RNF156.
Subcellular Location
Cytoplasm > Cytosol. Cell Membrane; Early Endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > Cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (92%), Bovine (92%), Horse (92%), Rabbit (92%), Dog (92%), Chicken (100%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemisry)
(AAA323136 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry)
(AAA323136 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using RNF156 antibody. Lane 1: mouse brain treated with blocking peptide. Lane 2: Mouse brain; Lane 3: 3T3; Lane 4: Hela;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using RNF156 antibody. Lane 1: mouse brain treated with blocking peptide. Lane 2: Mouse brain; Lane 3: 3T3; Lane 4: Hela;)
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using RNF156 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using RNF156 antibody.)
Related Product Information for anti-RNF156 antibody
Function: E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R-and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity).
Subunit Structure: Interacts with MC1R and MC4R, but not with TBXA2R. Interacts with TSG101. Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement (By similarity).
Post-translational Modifications: Autoubiquitinated in vitro.
Similarity: The RING finger is required for ubiquitin ligase activity.
Subunit Structure: Interacts with MC1R and MC4R, but not with TBXA2R. Interacts with TSG101. Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement (By similarity).
Post-translational Modifications: Autoubiquitinated in vitro.
Similarity: The RING finger is required for ubiquitin ligase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 66-72 kDa; 55 kDa
Predicted: 61 kDa
Predicted: 61 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase MGRN1 isoform 2
NCBI Official Synonym Full Names
mahogunin ring finger 1
NCBI Official Symbol
MGRN1
NCBI Official Synonym Symbols
RNF156
NCBI Protein Information
E3 ubiquitin-protein ligase MGRN1
UniProt Protein Name
E3 ubiquitin-protein ligase MGRN1
UniProt Gene Name
MGRN1
UniProt Synonym Gene Names
KIAA0544; RNF156
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Product Notes
The RNF156 mgrn1 (Catalog #AAA323136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF156 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF156 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RNF156 mgrn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF156, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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