WB (Western Blot)
(Detection of human RNF25 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody AAA212311 (lot AAA212311-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human RNF25 Polyclonal Antibody | anti-RNF25 antibody
Rabbit anti-RNF25 Antibody, Affinity Purified
Gene Names
RNF25; AO7
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RNF25, Antibody; Rabbit anti-RNF25 Antibody, Affinity Purified; ring finger protein AO7; RING-type E3 ubiquitin transferase RNF25; E3 ubiquitin-protein ligase RNF25; AO7; RING finger protein 25; ring finger protein 25; anti-RNF25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RNF25 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 409 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RNF25 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody AAA212311 (lot AAA212311-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human RNF25 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody AAA212311 (lot AAA212311-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RNF25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody AAA212311 (lot AAA212311-1) used for IP at 6 ug/mg lysate. RNF25 was also immunoprecipitated by rabbit anti-RNF25 antibody BL13794. For blotting immunoprecipitated RNF25, AAA212311 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RNF25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody AAA212311 (lot AAA212311-1) used for IP at 6 ug/mg lysate. RNF25 was also immunoprecipitated by rabbit anti-RNF25 antibody BL13794. For blotting immunoprecipitated RNF25, AAA212311 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-RNF25 antibody
RNF25 (RING finger protein 25) is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (by similarity). RNF25 stimulates transcription mediated by NF-kappa-B [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BH1].
Product Categories/Family for anti-RNF25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,219 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF25
NCBI Official Synonym Full Names
ring finger protein 25
NCBI Official Symbol
RNF25
NCBI Official Synonym Symbols
AO7
NCBI Protein Information
E3 ubiquitin-protein ligase RNF25; ring finger protein AO7
UniProt Protein Name
E3 ubiquitin-protein ligase RNF25
UniProt Gene Name
RNF25
UniProt Entry Name
RNF25_HUMAN
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Product Notes
The RNF25 rnf25 (Catalog #AAA212311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RNF25 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF25 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RNF25 rnf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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