WB (Western Blot)
(RNF4 Antibody (C-term) western blot analysis in LNCaP cell line lysates (35ug/lane).This demonstrates the RNF4 antibody detected the RNF4 protein (arrow).)
Rabbit anti-Human RNF4 Polyclonal Antibody | anti-RNF4 antibody
RNF4 Antibody (C-term)
Gene Names
RNF4; SLX5; SNURF; RES4-26
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF4, Antibody; RNF4 Antibody (C-term); E3 ubiquitin-protein ligase RNF4; 632-; RING finger protein 4; Small nuclear ring finger protein; Protein SNURF; RNF4; SNURF; anti-RNF4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RNF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the C-terminal region of human RNF4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
95-123
Sequence Length
190
Applicable Applications for anti-RNF4 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(RNF4 Antibody (C-term) western blot analysis in LNCaP cell line lysates (35ug/lane).This demonstrates the RNF4 antibody detected the RNF4 protein (arrow).)
WB (Western Blot)
(RNF4 Antibody (C-term) western blot analysis in LNCaP cell line lysates (35ug/lane).This demonstrates the RNF4 antibody detected the RNF4 protein (arrow).)
WB (Western Blot)
(Anti-RNF4 Antibody (C-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-RNF4 Antibody (C-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RNF4 antibody
The protein encoded by this gene contains a RING finger
motif and acts as a transcription regulator. This protein has been
shown to interact with, and inhibit the activity of, TRPS1, a
transcription suppressor of GATA-mediated transcription.
Transcription repressor ZNF278/PATZ is found to interact with this
protein, and thus reduce the enhancement of androgen
receptor-dependent transcription mediated by this protein. Studies
of the mouse and rat counterparts suggested a role of this protein
in spermatogenesis. A pseudogene of this gene is found on
chromosome 1.
motif and acts as a transcription regulator. This protein has been
shown to interact with, and inhibit the activity of, TRPS1, a
transcription suppressor of GATA-mediated transcription.
Transcription repressor ZNF278/PATZ is found to interact with this
protein, and thus reduce the enhancement of androgen
receptor-dependent transcription mediated by this protein. Studies
of the mouse and rat counterparts suggested a role of this protein
in spermatogenesis. A pseudogene of this gene is found on
chromosome 1.
Product Categories/Family for anti-RNF4 antibody
References
Hu, X.V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15087-15092(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Salonen, J., et al. Mol. Cell. Endocrinol. 307 (1-2), 205-210 (2009) :
Percherancier, Y., et al. J. Biol. Chem. 284(24):16595-16608(2009)
Tatham, M.H., et al. Nat. Cell Biol. 10(5):538-546(2008)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Salonen, J., et al. Mol. Cell. Endocrinol. 307 (1-2), 205-210 (2009) :
Percherancier, Y., et al. J. Biol. Chem. 284(24):16595-16608(2009)
Tatham, M.H., et al. Nat. Cell Biol. 10(5):538-546(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21319
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF4 isoform 1
NCBI Official Synonym Full Names
ring finger protein 4
NCBI Official Symbol
RNF4
NCBI Official Synonym Symbols
SLX5; SNURF; RES4-26
NCBI Protein Information
E3 ubiquitin-protein ligase RNF4
UniProt Protein Name
E3 ubiquitin-protein ligase RNF4
UniProt Gene Name
RNF4
UniProt Synonym Gene Names
SNURF; Protein SNURF
UniProt Entry Name
RNF4_HUMAN
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Product Notes
The RNF4 rnf4 (Catalog #AAA286476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 95-123. The RNF4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RNF4 rnf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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