IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA233140 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human ROBO1 Polyclonal Antibody | anti-ROBO1 antibody
ROBO1 Antibody
Gene Names
ROBO1; SAX3; DUTT1
Reactivity
Human
Applications
ELISA
Purity
>95%, Protein G purified
Synonyms
ROBO1, Antibody; ROBO1 Antibody; Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1; anti-ROBO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
1551
Applicable Applications for anti-ROBO1 antibody
ELISA
Immunogen
Recombinant Human Roundabout homolog 1 protein (29-143AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-16611 / sc-16612 / sc-25672
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA233140 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA233140 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: ROBO1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 181,182,177,176,170 KDaObserved band size: 181 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: ROBO1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 181,182,177,176,170 KDaObserved band size: 181 KDa)
Related Product Information for anti-ROBO1 antibody
Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed: 10102268, PubMed: 24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed: 24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed: 26529257). May be required for lung development (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
169,661 Da
NCBI Official Full Name
roundabout homolog 1 isoform d
NCBI Official Synonym Full Names
roundabout guidance receptor 1
NCBI Official Symbol
ROBO1
NCBI Official Synonym Symbols
SAX3; DUTT1
NCBI Protein Information
roundabout homolog 1
UniProt Protein Name
Roundabout homolog 1
UniProt Gene Name
ROBO1
UniProt Synonym Gene Names
DUTT1
UniProt Entry Name
ROBO1_HUMAN
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Product Notes
The ROBO1 robo1 (Catalog #AAA233140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROBO1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ROBO1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the ROBO1 robo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROBO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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