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product-image-AAA12322_WB2.jpg WB (Western Blot) (Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ROBO1 Polyclonal Antibody | anti-ROBO1 antibody

Goat Polyclonal to Human ROBO1

Gene Names
ROBO1; SAX3; DUTT1
Reactivity
Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Synonyms
ROBO1, Antibody; Goat Polyclonal to Human ROBO1; Anti-ROBO1 Antibody (Internal) IHC-plus; ROBO1; Deleted in U twenty twenty; H-Robo-1; Roundabout 1; SAX3; DUTT1; Roundabout homolog 1; Human ROBO1; anti-ROBO1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Human ROBO1. This antibody is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
1651
Applicable Applications for anti-ROBO1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
IHC-P: 2 ug/ml
WB: 1 ug/ml
IF: 10 ug/ml
Peptide Enzyme-Linked Immunoabsorbent Assay: 1:8000

Optimal dilutions to be determined by the researcher.
Target
ROBO1
Immunogen
ROBO1 antibody was raised against peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1; NP_598334.1.
Immunogen Type
ROBO1 antibody was raised against Human.
Epitope
aa994-1006
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.

WB (Western Blot)

(Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

product-image-AAA12322_WB2.jpg WB (Western Blot) (Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry)

(Anti-ROBO1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

product-image-AAA12322_IHC.jpg IHC (Immunohistochemistry) (Anti-ROBO1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)
Related Product Information for anti-ROBO1 antibody
Immunohistochemistry: MBS242360 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242360 was determined to be 2 ug/ml.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
roundabout homolog 1 isoform a
NCBI Official Synonym Full Names
roundabout, axon guidance receptor, homolog 1 (Drosophila)
NCBI Official Symbol
ROBO1
NCBI Official Synonym Symbols
SAX3; DUTT1
NCBI Protein Information
roundabout homolog 1; deleted in U twenty twenty
UniProt Protein Name
Roundabout homolog 1
UniProt Gene Name
ROBO1
UniProt Synonym Gene Names
DUTT1
UniProt Entry Name
ROBO1_HUMAN

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Product Notes

The ROBO1 robo1 (Catalog #AAA12322) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human ROBO1 reacts with Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ROBO1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). IHC-P: 2 ug/ml WB: 1 ug/ml IF: 10 ug/ml Peptide Enzyme-Linked Immunoabsorbent Assay: 1:8000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ROBO1 robo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROBO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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