FCM/FACS (Flow Cytometry)
(Roundabout 4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human Roundabout 4 Polyclonal Antibody | anti-ROBO4 antibody
Roundabout 4 Antibody (N-term)
Gene Names
ROBO4; MRB; ECSM4
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Roundabout 4, Antibody; Roundabout 4 Antibody (N-term); Roundabout homolog 4; Magic roundabout; ROBO4; anti-ROBO4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
236-264
Sequence Length
702
Applicable Applications for anti-ROBO4 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Roundabout 4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Roundabout 4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Roundabout 4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Roundabout 4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Roundabout 4 Antibody (N-term) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).)
WB (Western Blot)
(Roundabout 4 Antibody (N-term) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).)
Related Product Information for anti-ROBO4 antibody
Roundabout 4 is receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. This protein may mediate the inhibition of primary endothelial cell migration by Slit proteins.
Product Categories/Family for anti-ROBO4 antibody
References
Jones, C.A., et al. Nat. Cell Biol. 11(11):1325-1331(2009)
Sheldon, H., et al. FASEB J. 23(2):513-522(2009)
Huang, L., et al. Mol. Vis. 15, 1057-1069 (2009)
Sheldon, H., et al. FASEB J. 23(2):513-522(2009)
Huang, L., et al. Mol. Vis. 15, 1057-1069 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107457
NCBI Official Full Name
roundabout homolog 4 isoform 1
NCBI Official Synonym Full Names
roundabout guidance receptor 4
NCBI Official Symbol
ROBO4
NCBI Official Synonym Symbols
MRB; ECSM4
NCBI Protein Information
roundabout homolog 4
UniProt Protein Name
Roundabout homolog 4
UniProt Gene Name
ROBO4
UniProt Entry Name
ROBO4_HUMAN
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Product Notes
The ROBO4 robo4 (Catalog #AAA283842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-264. The Roundabout 4 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Roundabout 4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ROBO4 robo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Roundabout 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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