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product-image-AAA200612_WB10.jpg WB (Western Blot) (WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

Rabbit RPA1 Polyclonal Antibody | anti-RPA1 antibody

RPA1 antibody - middle region

Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
Applications
Western Blot
Purity
Affinity Purified
Synonyms
RPA1, Antibody; RPA1 antibody - middle region; anti-RPA1 antibody
Ordering
Host
Rabbit
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: TLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLSVLSSSTIIANPDIPEA
Sequence Length
616
Applicable Applications for anti-RPA1 antibody
WB (Western Blot)
Protein Size (# AA)
616 amino acids
Protein Interactions
STRA13; UBC; MTUS2; APITD1; PCNA; SUMO2; FBXO18; ATRX; MAP4; CAPRIN1; LMO7; LIG3; LDHA; ITGB1; IMPDH2; ILF3; ILF2; HSPB1; HSPA8; HSPA5; HNRNPU; HNRNPL; HNRNPK; HNRNPH1; HNRNPF; HNRNPD; HNRNPAB; HNRNPA2B1; HNRNPA1; HCFC1; GTF3C2; GTF2I; GTF2H3; GAPDH; XRCC
Blocking Peptide
For anti-RPA1 (MBS3212832) antibody is Catalog # MBS3237778
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human RPA1
Replacement Item
This antibody may replace item sc-14696 from Santa Cruz Biotechnology.
Predicted Homology
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 93%
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

product-image-AAA200612_WB10.jpg WB (Western Blot) (WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

WB (Western Blot)

(Host: RabbitTarget Name: RPA1Sample Type: Human MCF7Antibody Dilution: 1.0ug/mlRPA1 is supported by BioGPS gene expression data to be expressed in MCF7)

product-image-AAA200612_WB11.jpg WB (Western Blot) (Host: RabbitTarget Name: RPA1Sample Type: Human MCF7Antibody Dilution: 1.0ug/mlRPA1 is supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot)

(Host: RabbitTarget Name: RPA1Sample Type: Human HelaAntibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

product-image-AAA200612_WB13.jpg WB (Western Blot) (Host: RabbitTarget Name: RPA1Sample Type: Human HelaAntibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot)

(Host: RabbitTarget Name: RPA1Sample Type: Human 721_BAntibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

product-image-AAA200612_WB15.jpg WB (Western Blot) (Host: RabbitTarget Name: RPA1Sample Type: Human 721_BAntibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
Related Product Information for anti-RPA1 antibody
This is a rabbit polyclonal antibody against RPA1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. RPA1 binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68kDa
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit isoform 1
NCBI Official Synonym Full Names
replication protein A1
NCBI Official Symbol
RPA1
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Gene Name
RPA1
UniProt Synonym Gene Names
REPA1; RPA70; RP-A p70
UniProt Entry Name
RFA1_HUMAN

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Product Notes

The RPA1 rpa1 (Catalog #AAA200612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPA1 antibody - middle region reacts with Tested Reactivity: Human Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's RPA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the RPA1 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: TLWGEDADKF DGSRQPVLAI KGARVSDFGG RSLSVLSSST IIANPDIPEA. It is sometimes possible for the material contained within the vial of "RPA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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