IHC (Immunohistochemisry)
(Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human RPA3 Polyclonal Antibody | anti-RPA3 antibody
Anti-RPA3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
RPA3, Antibody; Anti-RPA3 Antibody, Rabbit Polyclonal; RPA3 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-REPA3 Antibody; Anti-RP-A p14 Antibody; anti-RPA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RPA3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RPA3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human RPA3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RPA3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemisry)
(Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human RPA3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human RPA3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-RPA3 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
WB (Western Blot)
(Anti-RPA3 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
Related Product Information for anti-RPA3 antibody
The replication protein A3 (RPA3) gene is located on chromosome 7p22. RPA3 is a component of the RPA protein complex, which plays an essential role in multiple processes of DNA metabolism. RPA3 is upregulated in hepatocellular carcinoma (HCC) and gastric cancer tissues. High expression of RPA3 is associated with poorer patient survival. RPA3 represents a new therapeutic target of gastric cancer as well as hepatocellular carcinoma and serves as a potential prognostic biomarker for patient survival.
References
Qu C, et al. (2017) Rpa3 is a potential marker of prognosis and radioresistance for nasopharyngeal carcinoma. J Cell Mol Med 21 (11): 2872-2883.Jin T, et al. (2013) Tp53 and rpa3 gene variations were associated with risk of glioma in a chinese han population. Cancer Biother Radiopharm 28 (3): 248-253.Dai Z, et al. (2017) Elevated expression of rpa3 is involved in gastric cancer tumorigenesis and associated with poor patient survival. Dig Dis Sci 62 (9): 2369-2375.
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Product Notes
The RPA3 (Catalog #AAA256406) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPA3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RPA3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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