WB (Western Blot)
(Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (AAA210838 lot 9) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse RPA32 Polyclonal Antibody | anti-RPA32 antibody
Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (AAA210838 lot 9) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (AAA210838 lot 9) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with or without etoposide. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (AAA210838 lot 9) used for IP at 6 ug per reaction followed by treatment with Lambda phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho RPA32 (S4/S8), AAA210838 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPA32 antibody .)
IP (Immunoprecipitation)
(Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with or without etoposide. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (AAA210838 lot 9) used for IP at 6 ug per reaction followed by treatment with Lambda phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho RPA32 (S4/S8), AAA210838 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPA32 antibody .)
IHC (Immunohistochemisry)
(Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse gut, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) ((AAA210838 lot 9)) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemisry)
(Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse gut, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) ((AAA210838 lot 9)) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human breast carcinoma, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) ((AAA210838 lot 9)) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human breast carcinoma, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) ((AAA210838 lot 9)) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
ICC (Immunocytochemistry)
(Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (Cat. No. AAA210838 lot 4) used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor 488 phalloidin (green).)
ICC (Immunocytochemistry)
(Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (Cat. No. AAA210838 lot 4) used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor 488 phalloidin (green).)
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Product Notes
The RPA32 rpa2 (Catalog #AAA210838) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPA32 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPA32 rpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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