IHC (Immunohiostchemistry)
(AAA327964 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit RPAB3 Polyclonal Antibody | anti-RPAB3 antibody
RPAB3 Antibody
Gene Names
POLR2H; RPB8; RPB17; RPABC3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RPAB3, Antibody; RPAB3 Antibody; and III 17.1 kDa polypeptide; and III subunit ABC3; DNA directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA directed RNA polymerases I, II, and III subunit RPABC3; DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I; DNA-directed RNA polymerases I, II, and III subunit RPABC3; hRPB8; HsRPB8; II; POLR2H; Polymerase (RNA) II (DNA directed) polypeptide H; RNA polymerases I; RNA polymerases I, II, and III subunit ABC3; RPAB3_HUMAN; RPABC3; RPB17; RPB8; RPB8 homolog; anti-RPAB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RPAB3 antibody detects endogenous levels of total RPAB3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
175
Applicable Applications for anti-RPAB3 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human RPAB3
Subcellular Location
Nucleus > Nucleolus.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327964 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327964 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of RPAB3 using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of RPAB3 using HeLa whole cell lysates)
Related Product Information for anti-RPAB3 antibody
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
Subunit Structure: Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. Directly interacts with POLR2A.
Similarity: Belongs to the eukaryotic RPB8 RNA polymerase subunit family.
Subunit Structure: Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. Directly interacts with POLR2A.
Similarity: Belongs to the eukaryotic RPB8 RNA polymerase subunit family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 17 kDa
Predicted: 18 kDa
Predicted: 18 kDa
NCBI Official Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 1
NCBI Official Synonym Full Names
RNA polymerase II subunit H
NCBI Official Symbol
POLR2H
NCBI Official Synonym Symbols
RPB8; RPB17; RPABC3
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC3
UniProt Protein Name
DNA-directed RNA polymerases I, II, and III subunit RPABC3
UniProt Gene Name
POLR2H
UniProt Synonym Gene Names
RNA polymerases I, II, and III subunit ABC3; hRPB8
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Product Notes
The RPAB3 polr2h (Catalog #AAA327964) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPAB3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPAB3 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RPAB3 polr2h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPAB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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