WB (Western Blot)
(Western BlotPositive WB detected in: MCF-7 whole cell lysateAll lanes: RPE65 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 kDaObserved band size: 61 kDa)
Rabbit anti-Human, Rat RPE65 Polyclonal Antibody | anti-RPE65 antibody
RPE65 Antibody
Gene Names
RPE65; p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65
Reactivity
Human, Rat
Applications
Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
RPE65, Antibody; RPE65 Antibody; Retinoid isomerohydrolase (EC 3.1.1.64) (All-trans-retinyl-palmitate hydrolase) (Meso-zeaxanthin isomerase) (EC 5.3.3.-) (Retinal pigment epithelium-specific 65 kDa protein) (Retinol isomerase), RPE65 ; anti-RPE65 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid; Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Applicable Applications for anti-RPE65 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
Recombinant Human Retinoid isomerohydrolase protein (348-484AA)
Conjugate
Non-conjugated
Species
Homo sapiens (Human)
Research Area
Tags & Cell Markers
Target Names
RPE65
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
WB (Western Blot)
(Western BlotPositive WB detected in: MCF-7 whole cell lysateAll lanes: RPE65 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 kDaObserved band size: 61 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: MCF-7 whole cell lysateAll lanes: RPE65 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 kDaObserved band size: 61 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA234638 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA234638 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: Rat brain tissueAll lanes: RPE65 antibody at 2.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 KDaObserved band size: 61 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Rat brain tissueAll lanes: RPE65 antibody at 2.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 KDaObserved band size: 61 KDa)
Related Product Information for anti-RPE65 antibody
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
Product Categories/Family for anti-RPE65 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
retinoid isomerohydrolase
NCBI Official Synonym Full Names
RPE65, retinoid isomerohydrolase
NCBI Official Symbol
RPE65
NCBI Official Synonym Symbols
p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65
NCBI Protein Information
retinoid isomerohydrolase
UniProt Protein Name
Retinoid isomerohydrolase
UniProt Gene Name
RPE65
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Product Notes
The RPE65 rpe65 (Catalog #AAA234638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPE65 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPE65 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RPE65 rpe65 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPE65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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