WB (Western Blot)
(Detection of human and mouse RPL21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL21 antibody AAA213067 (lot AAA213067-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human, Mouse RPL21/Ribosomal Protein L21 Polyclonal Antibody | anti-RPL21 antibody
Rabbit anti-RPL21/Ribosomal Protein L21 Antibody, Affinity Purified
Gene Names
RPL21; L21; HYPT12
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RPL21/Ribosomal Protein L21, Antibody; Rabbit anti-RPL21/Ribosomal Protein L21 Antibody, Affinity Purified; HYPT12; L21; 60S ribosomal protein L21; large ribosomal subunit protein eL21; ribosomal protein L21; anti-RPL21 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RPL21 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Goat
Immunogen
Between 110 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RPL21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL21 antibody AAA213067 (lot AAA213067-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse RPL21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL21 antibody AAA213067 (lot AAA213067-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human RPL21 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL21 antibody AAA213067 (lot AAA213067-1) used for IP at 6 ug per reaction. RPL21 was also immunoprecipitated by rabbit anti-RPL21 antibody For blotting immunoprecipitated RPL21, AAA213067 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human RPL21 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL21 antibody AAA213067 (lot AAA213067-1) used for IP at 6 ug per reaction. RPL21 was also immunoprecipitated by rabbit anti-RPL21 antibody For blotting immunoprecipitated RPL21, AAA213067 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-RPL21 antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL21/Ribosomal Protein L21 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6144)].
Product Categories/Family for anti-RPL21 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,565 Da
NCBI Official Full Name
60S ribosomal protein L21
NCBI Official Synonym Full Names
ribosomal protein L21
NCBI Official Symbol
RPL21
NCBI Official Synonym Symbols
L21; HYPT12
NCBI Protein Information
60S ribosomal protein L21
UniProt Protein Name
60S ribosomal protein L21
UniProt Gene Name
RPL21
UniProt Entry Name
RL21_HUMAN
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Product Notes
The RPL21 rpl21 (Catalog #AAA213067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPL21/Ribosomal Protein L21 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPL21/Ribosomal Protein L21 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPL21 rpl21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL21/Ribosomal Protein L21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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