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FCM/FACS (Flow Cytometry)
(RPL27A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit RPL27A Polyclonal Antibody | anti-RPL27A antibody
RPL27A Antibody (C-term)
Gene Names
RPL27A; L27A
Reactivity
Human, hamster (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPL27A, Antibody; RPL27A Antibody (C-term); 60S ribosomal protein L27a; RPL27A; anti-RPL27A antibody
Host
Rabbit
Reactivity
Human, hamster (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
110-138
Sequence Length
148
Applicable Applications for anti-RPL27A antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RPL27A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RPL27A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(RPL27A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(RPL27A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(RPL27A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)
WB (Western Blot)
(RPL27A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)
WB (Western Blot)
(RPL27A Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)
WB (Western Blot)
(RPL27A Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)
Related Product Information for anti-RPL27A antibody
Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15P family of ribosomal proteins. It is located in the
cytoplasm. Variable expression of this gene in colorectal cancers
compared to adjacent normal tissues has been observed, although no
correlation between the level of expression and the severity of the
disease has been found. As is typical for genes encoding ribosomal
proteins, multiple processed pseudogenes derived from this gene are
dispersed through the genome.
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15P family of ribosomal proteins. It is located in the
cytoplasm. Variable expression of this gene in colorectal cancers
compared to adjacent normal tissues has been observed, although no
correlation between the level of expression and the severity of the
disease has been found. As is typical for genes encoding ribosomal
proteins, multiple processed pseudogenes derived from this gene are
dispersed through the genome.
References
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)
Bortoluzzi, S., et al. Bioinformatics 17(12):1152-1157(2001)
Kusuda, J., et al. Cytogenet. Cell Genet. 85 (3-4), 248-251 (1999) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)
Bortoluzzi, S., et al. Bioinformatics 17(12):1152-1157(2001)
Kusuda, J., et al. Cytogenet. Cell Genet. 85 (3-4), 248-251 (1999) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16561
NCBI Official Full Name
60S ribosomal protein L27a
NCBI Official Synonym Full Names
ribosomal protein L27a
NCBI Official Symbol
RPL27A
NCBI Official Synonym Symbols
L27A
NCBI Protein Information
60S ribosomal protein L27a
UniProt Protein Name
60S ribosomal protein L27a
UniProt Gene Name
RPL27A
UniProt Entry Name
RL27A_HUMAN
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Product Notes
The RPL27A rpl27a (Catalog #AAA284256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 110-138. The RPL27A Antibody (C-term) reacts with Human, hamster (Predicted Reactivity: Bovine, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RPL27A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RPL27A rpl27a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL27A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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