<
WB (Western Blot)
(Detection of human and mouse RPL5 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL5 antibody AAA212386 (lot AAA212386-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RPL5 Polyclonal Antibody | anti-RPL5 antibody
Rabbit anti-RPL5 Antibody, Affinity Purified
Gene Names
RPL5; L5; DBA6; MSTP030
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RPL5, Antibody; Rabbit anti-RPL5 Antibody, Affinity Purified; 60S ribosomal protein L5; PPP1R135; protein phosphatase 1, regulatory subunit 135; uL18; L5; large ribosomal subunit protein uL18; MSTP030; ribosomal protein L5; anti-RPL5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RPL5 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Rabbit
Immunogen
Between 247 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RPL5 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL5 antibody AAA212386 (lot AAA212386-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse RPL5 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL5 antibody AAA212386 (lot AAA212386-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPL5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody AAA212386 (lot AAA212386-3) used for IP at 3 ug per reaction. RPL5 was also immunoprecipitated by rabbit anti-RPL5 antibody BL14170. For blotting immunoprecipitated RPL5, AAA212386 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPL5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody AAA212386 (lot AAA212386-3) used for IP at 3 ug per reaction. RPL5 was also immunoprecipitated by rabbit anti-RPL5 antibody BL14170. For blotting immunoprecipitated RPL5, AAA212386 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. AAA212386 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. AAA212386 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. AAA212386 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. AAA212386 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-RPL5 antibody
Ribosomal protein L5 (RPL5) is required for rRNA maturation and formation of the 60S ribosomal subunits. RPL5 binds 5S RNA. Defects in the RPL5 gene are the cause of Diamond-Blackfan anemia type 6 (DBA6), a congenital non-regenerative hypoplastic anemia that usually presents early in infancy [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46777].
Product Categories/Family for anti-RPL5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,363 Da
NCBI Official Full Name
60S ribosomal protein L5
NCBI Official Synonym Full Names
ribosomal protein L5
NCBI Official Symbol
RPL5
NCBI Official Synonym Symbols
L5; DBA6; MSTP030
NCBI Protein Information
60S ribosomal protein L5
UniProt Protein Name
60S ribosomal protein L5
UniProt Gene Name
RPL5
UniProt Entry Name
RL5_HUMAN
Similar Products
Product Notes
The RPL5 rpl5 (Catalog #AAA212386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPL5 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPL5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPL5 rpl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.