WB (Western Blot)
(Detection of human and mouse RPL8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL8 antibody AAA213079 (lot AAA213079-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RPL8/Ribosomal Protein L8 Polyclonal Antibody | anti-RPL8 antibody
Rabbit anti-RPL8/Ribosomal Protein L8 Antibody, Affinity Purified
Gene Names
RPL8; L8
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RPL8/Ribosomal Protein L8, Antibody; Rabbit anti-RPL8/Ribosomal Protein L8 Antibody, Affinity Purified; 60S ribosomal protein L8; L8; large ribosomal subunit protein uL2; ribosomal protein L8; anti-RPL8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RPL8 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Zebrafish, X. laevis, X. tropicalis, Bovine, Orangutan
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RPL8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL8 antibody AAA213079 (lot AAA213079-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse RPL8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL8 antibody AAA213079 (lot AAA213079-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPL8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL8 antibody AAA213079 (lot AAA213079-1) used for IP at 6 ug per reaction. RPL8 was also immunoprecipitated by rabbit anti-RPL8 antibody For blotting immunoprecipitated RPL8, AAA213079 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RPL8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL8 antibody AAA213079 (lot AAA213079-1) used for IP at 6 ug per reaction. RPL8 was also immunoprecipitated by rabbit anti-RPL8 antibody For blotting immunoprecipitated RPL8, AAA213079 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-RPL8 antibody
The gene RPL8 encodes 60S ribosomal protein L8 that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins and is located in the cytoplasm. In rat, the protein associates with the 5. 8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface [taken from NCBI Entrez Gene (Gene ID: 6132)].
Product Categories/Family for anti-RPL8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,025 Da
NCBI Official Full Name
60S ribosomal protein L8
NCBI Official Synonym Full Names
ribosomal protein L8
NCBI Official Symbol
RPL8
NCBI Official Synonym Symbols
L8
NCBI Protein Information
60S ribosomal protein L8
UniProt Protein Name
60S ribosomal protein L8
UniProt Gene Name
RPL8
UniProt Entry Name
RL8_HUMAN
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Product Notes
The RPL8 rpl8 (Catalog #AAA213079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPL8/Ribosomal Protein L8 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPL8/Ribosomal Protein L8 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPL8 rpl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL8/Ribosomal Protein L8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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