FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit RPS15 Polyclonal Antibody | anti-RPS15 antibody
RPS15 Antibody (N-term)
Gene Names
RPS15; RIG; S15
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPS15, Antibody; RPS15 Antibody (N-term); 40S ribosomal protein S15; RIG protein; RPS15; RIG; anti-RPS15 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPS15.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
145
Applicable Applications for anti-RPS15 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of RPS15 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of RPS15 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.)
Related Product Information for anti-RPS15 antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS15 is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm.
References
Yu,Y., et.al., Protein Sci. 14 (6), 1438-1446 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17040
NCBI Official Full Name
40S ribosomal protein S15 isoform 2
NCBI Official Synonym Full Names
ribosomal protein S15
NCBI Official Symbol
RPS15
NCBI Official Synonym Symbols
RIG; S15
NCBI Protein Information
40S ribosomal protein S15
UniProt Protein Name
40S ribosomal protein S15
UniProt Gene Name
RPS15
UniProt Synonym Gene Names
RIG
UniProt Entry Name
RS15_HUMAN
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Product Notes
The RPS15 rps15 (Catalog #AAA288036) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The RPS15 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RPS15 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RPS15 rps15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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