FCM/FACS (Flow Cytometry)
(RPS6 Antibody (Ser240/244) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit RPS6 (Ser240/244) Polyclonal Antibody | anti-RPS6 antibody
RPS6 (Ser240/244) Antibody
Gene Names
RPS6; S6
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Monkey, Mouse, Rat, Xenopus)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPS6 (Ser240/244), Antibody; RPS6 (Ser240/244) Antibody; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6; anti-RPS6 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Monkey, Mouse, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
220-249
Sequence Length
249
Applicable Applications for anti-RPS6 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RPS6 Antibody (Ser240/244) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RPS6 Antibody (Ser240/244) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(RPS6 antibody (Ser240/244) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(RPS6 antibody (Ser240/244) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244). AAA288858 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244). AAA288858 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-RPS6 antibody
Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a cytoplasmic
ribosomal protein that is a component of the 40S subunit. The
protein belongs to the S6E family of ribosomal proteins. It is the
major substrate of protein kinases in the ribosome, with subsets of
five C-terminal serine residues phosphorylated by different protein
kinases. Phosphorylation is induced by a wide range of stimuli,
including growth factors, tumor-promoting agents, and mitogens.
Dephosphorylation occurs at growth arrest. The protein may
contribute to the control of cell growth and proliferation through
the selective translation of particular classes of mRNA. As is
typical for genes encoding ribosomal proteins, there are multiple
processed pseudogenes of this gene dispersed through the genome.
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a cytoplasmic
ribosomal protein that is a component of the 40S subunit. The
protein belongs to the S6E family of ribosomal proteins. It is the
major substrate of protein kinases in the ribosome, with subsets of
five C-terminal serine residues phosphorylated by different protein
kinases. Phosphorylation is induced by a wide range of stimuli,
including growth factors, tumor-promoting agents, and mitogens.
Dephosphorylation occurs at growth arrest. The protein may
contribute to the control of cell growth and proliferation through
the selective translation of particular classes of mRNA. As is
typical for genes encoding ribosomal proteins, there are multiple
processed pseudogenes of this gene dispersed through the genome.
Product Categories/Family for anti-RPS6 antibody
References
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008)
Robledo, S., et al. RNA 14(9):1918-1929(2008)
Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008) :
Ma, X.M., et al. Cell 133(2):303-313(2008)
Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008)
Robledo, S., et al. RNA 14(9):1918-1929(2008)
Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008) :
Ma, X.M., et al. Cell 133(2):303-313(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28681
NCBI Official Full Name
40S ribosomal protein S6
NCBI Official Synonym Full Names
ribosomal protein S6
NCBI Official Symbol
RPS6
NCBI Official Synonym Symbols
S6
NCBI Protein Information
40S ribosomal protein S6
UniProt Protein Name
40S ribosomal protein S6
UniProt Gene Name
RPS6
UniProt Entry Name
RS6_HUMAN
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Product Notes
The RPS6 rps6 (Catalog #AAA288858) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 220-249. The RPS6 (Ser240/244) Antibody reacts with Human (Predicted Reactivity: Bovine, Chicken, Monkey, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6 (Ser240/244) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the RPS6 rps6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6 (Ser240/244), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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