Rabbit anti-Human, mouse RRAGD Polyclonal Antibody | anti-RRAGD antibody

RRAGD Antibody (Center)

Cat. Num. AAA283875

• Gene Names: RRAGD; RAGD; bA11D8.2.1
• Reactivity: Human, mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: RRAGD, Antibody; RRAGD Antibody (Center); Ras-related GTP-binding protein D; Rag D; RagD; RRAGD HGNC:19903); anti-RRAGD antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 171-199
• Sequence Length: 249

• Applicable Applications for anti-RRAGD antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed:20381137, PubMed:24095279). Forms heterodimeric Rag complexes with RRAGA or RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form (PubMed:24095279). In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed:20381137, PubMed:24095279). This is a crucial step in the activation of the TOR signaling cascade by amino acids (PubMed:20381137, PubMed:24095279).
• Cellular Location: Cytoplasm. Nucleus. Lysosome Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA (PubMed:11073942).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(RRAGD Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(RRAGD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-RRAGD Antibody (Center) at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Western blot analysis of RRAGD Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of RRAGD Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.)

Related Product Information for anti-RRAGD antibody

RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.

Product Categories/Family for anti-RRAGD antibody

Signal Transduction

References

Olsen, J.V., et al. Cell 127(3):635-648(2006)
de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005)
Sekiguchi, T., et al. J. Biol. Chem. 279(9):8343-8350(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 11034847
• NCBI GeneID: 58528
• NCBI Accession #: NP_067067.1
• NCBI GenBank Nucleotide #: NM_021244.4
• UniProt Accession #: Q9NQL2; Q7L8F9; Q9NPG0; A8K184
• Molecular Weight: 45588
• NCBI Official Full Name: ras-related GTP-binding protein D
• NCBI Official Synonym Full Names: Ras-related GTP binding D
• NCBI Official Symbol: RRAGD
• NCBI Official Synonym Symbols: RAGD; bA11D8.2.1
• NCBI Protein Information: ras-related GTP-binding protein D
• UniProt Protein Name: Ras-related GTP-binding protein D
• UniProt Gene Name: RRAGD
• UniProt Synonym Gene Names: Rag D; RagD
• UniProt Entry Name: RRAGD_HUMAN