IP (Immunoprecipitation)
(RPS6KA1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate1 uL anti-RPS6KA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RPS6KA1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 83 kDa)
Rabbit RSK1/RPS6KA1 Polyclonal Antibody | anti-RPS6KA1 antibody
Anti-RSK1/RPS6KA1 Antibody, Rabbit Polyclonal
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
RSK1/RPS6KA1, Antibody; Anti-RSK1/RPS6KA1 Antibody, Rabbit Polyclonal; RPS6KA1 Antibody, Rabbit PAb, Antigen Affinity Purified; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Anti-HU-1 Antibody; Anti-MAPKAPK1A Antibody; Anti-p90Rsk Antibody; Anti-RSK Antibody; Anti-RSK1 Antibody; anti-RPS6KA1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RSK1/RPS6KA1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-RPS6KA1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human RPS6KA1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human RPS6KA1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(RPS6KA1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate1 uL anti-RPS6KA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RPS6KA1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 83 kDa)
IP (Immunoprecipitation)
(RPS6KA1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate1 uL anti-RPS6KA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RPS6KA1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 83 kDa)
WB (Western Blot)
(Anti-RPS6KA1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:83 kDaObserved band size:83 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-RPS6KA1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:83 kDaObserved band size:83 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-RPS6KA1 antibody
RPS6KA1 (Ribosomal Protein S6 Kinase A1) is a Protein Coding gene. The RPS6KA1 gene, located on 1p36.11, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, C.elegans, and frog. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. RPS6KA1 is widely expressed in the small intestine, duodenum, and other tissues. Diseases associated with RPS6KA1 include Coffin-Lowry Syndrome and Tuberous Sclerosis. Among its related pathways are GDNF-Family Ligands and Receptor Interactions and NGF Pathway.
Product Categories/Family for anti-RPS6KA1 antibody
References
Salhi A, et al. (2015) Rsk1 activation promotes invasion in nodular melanoma. Am J Pathol 185 (3): 704-716.Li D, et al. (2012) Structural basis for the autoinhibition of the c-terminal kinase domain of human rsk1. Acta Crystallogr D Biol Crystallogr 68 (Pt 6): 680-685.Gogl G, et al. (2018) Dynamic control of rsk complexes by phosphoswitch-based regulation. Febs j 285 (1): 46-71.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,723 Da
NCBI Official Full Name
ribosomal protein S6 kinase alpha-1 isoform b
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol
RPS6KA1
NCBI Official Synonym Symbols
RSK; HU-1; RSK1; MAPKAPK1A
NCBI Protein Information
ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinas
UniProt Protein Name
Ribosomal protein S6 kinase alpha-1
UniProt Gene Name
RPS6KA1
UniProt Synonym Gene Names
MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
UniProt Entry Name
KS6A1_HUMAN
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Product Notes
The RPS6KA1 rps6ka1 (Catalog #AAA254632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RSK1/RPS6KA1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's RSK1/RPS6KA1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPS6KA1 rps6ka1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSK1/RPS6KA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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