WB (Western Blot)
(Detection of human Rtf1 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rtf1 antibody AAA210803 (lot AAA210803-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Rtf1 Polyclonal Antibody | anti-RTF1 antibody
Rabbit anti-Rtf1 Antibody, Affinity Purified
Gene Names
RTF1; GTL7; KIAA0252
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Rtf1, Antibody; Rabbit anti-Rtf1 Antibody, Affinity Purified; ortholog of mouse gene trap locus 7; RNA polymerase-associated protein RTF1 homolog; GTL7; Rtf1, Paf1/RNA polymerase II complex component, homolog; KIAA0252; RTF1 homolog, Paf1/RNA polymerase II complex component; anti-RTF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RTF1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 660 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Rtf1 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rtf1 antibody AAA210803 (lot AAA210803-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Rtf1 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rtf1 antibody AAA210803 (lot AAA210803-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Rtf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rtf1 antibody AAA210803 (lot AAA210803-1) used for IP at 3 ug per reaction. Rtf1 was also immunoprecipitated by rabbit anti-RTF1 antibodies and For blotting immunoprecipitated Rtf1, AAA210803 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Rtf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rtf1 antibody AAA210803 (lot AAA210803-1) used for IP at 3 ug per reaction. Rtf1 was also immunoprecipitated by rabbit anti-RTF1 antibodies and For blotting immunoprecipitated Rtf1, AAA210803 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-RTF1 antibody
In yeast, Rtf1 is a component of the Paf1/RNA polymerase II complex. The Paf1 complex interacts with RNA polymerase II and functions to modify chromatin during transcription. Rtf1 appears to be important for histone H2B ubiquitination by Rad6 and for methylation of histone H3 catalyzed by the Set1/COMPASS complex that functions during transcription elongation.
Product Categories/Family for anti-RTF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,313 Da
NCBI Official Full Name
RNA polymerase-associated protein RTF1 homolog
NCBI Official Synonym Full Names
Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
NCBI Official Symbol
RTF1
NCBI Official Synonym Symbols
GTL7; KIAA0252
NCBI Protein Information
RNA polymerase-associated protein RTF1 homolog; ortholog of mouse gene trap locus 7
UniProt Protein Name
RNA polymerase-associated protein RTF1 homolog
UniProt Gene Name
RTF1
UniProt Synonym Gene Names
KIAA0252
UniProt Entry Name
RTF1_HUMAN
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Product Notes
The RTF1 rtf1 (Catalog #AAA210803) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Rtf1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rtf1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RTF1 rtf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rtf1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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