IF (Immunofluorescence)
(Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit Ryanodine Receptor 2 (phospho-Ser2808) Polyclonal Antibody | anti-RYR2 antibody
Ryanodine Receptor 2 (phospho-Ser2808) Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity chromatography
Synonyms
Ryanodine Receptor 2 (phospho-Ser2808), Antibody; Ryanodine Receptor 2 (phospho-Ser2808) Antibody; Ryanodine receptor 2; RYR-2; RyR2; hRYR-2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor; anti-RYR2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of Ryanodine Receptor 2 (phospho-Ser2808) protein.
Purity/Purification
Immunogen affinity chromatography
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/mL (varies by lot)
Sequence Length
4967
Applicable Applications for anti-RYR2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:300
IHC: 1:50-1:100
IF: 1:100-1:300
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ryanodine Receptor 2.
Target Name
RYR2
Preparation and Storage
Store at -20°C.
IF (Immunofluorescence)
(Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of Ryanodine Receptor 2 (phospho-Ser2808) expression in U251 whole cell lysates.)
WB (Western Blot)
(Western blot analysis of Ryanodine Receptor 2 (phospho-Ser2808) expression in U251 whole cell lysates.)
Product Categories/Family for anti-RYR2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
564 KD
NCBI Official Full Name
ryanodine receptor 2
NCBI Official Synonym Full Names
ryanodine receptor 2
NCBI Official Symbol
RYR2
NCBI Official Synonym Symbols
RyR; ARVC2; ARVD2; RYR-2; VTSIP
NCBI Protein Information
ryanodine receptor 2
UniProt Protein Name
Ryanodine receptor 2
UniProt Gene Name
RYR2
UniProt Synonym Gene Names
RYR-2; RyR2; hRYR-2
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Product Notes
The RYR2 ryr2 (Catalog #AAA29761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ryanodine Receptor 2 (phospho-Ser2808) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ryanodine Receptor 2 (phospho-Ser2808) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:300. Researchers should empirically determine the suitability of the RYR2 ryr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ryanodine Receptor 2 (phospho-Ser2808), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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