IP (Immunoprecipitation)
(mS100A11 was immunoprecipitated using:Lane A:0.5 mg Mouse Liver Whole Cell Lysate4 uL anti-mS100A11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mS100A11 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size :11 kDa)
Rabbit anti-Mouse S100A11 Polyclonal Antibody | anti-S100A11 antibody
Anti-S100A11 Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
S100A11, Antibody; Anti-S100A11 Antibody, Rabbit Polyclonal; S100 calcium binding protein A11; Anti-cal Antibody; Anti-Emap1 Antibody; Anti-EMAPI Antibody; Anti-S100A11 Antibody; Anti-S100a14 Antibody; Anti-S100c Antibody; anti-S100A11 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse S100A11
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-S100A11 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse S100A11 Protein
Preparation
Produced in rabbits immunized with purified, recombinant Mouse S100A11 (rM S100A11). S100A11 specific IgG was purified by Mouse S100A11 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(mS100A11 was immunoprecipitated using:Lane A:0.5 mg Mouse Liver Whole Cell Lysate4 uL anti-mS100A11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mS100A11 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size :11 kDa)
IP (Immunoprecipitation)
(mS100A11 was immunoprecipitated using:Lane A:0.5 mg Mouse Liver Whole Cell Lysate4 uL anti-mS100A11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mS100A11 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size :11 kDa)
WB (Western Blot)
(Anti-S100A11 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse Liver Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:11 kDaObserved band size:11 kDa)
WB (Western Blot)
(Anti-S100A11 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse Liver Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:11 kDaObserved band size:11 kDa)
Related Product Information for anti-S100A11 antibody
Protein S100-A11, also known as S100 calcium-binding protein A11, S100A11 and MLN70, is a member of theS-100 family. S100A11 is widely expressed in multiple tissues, and is located in cytoplasm, nucleus, and even cell periphery. S100A11 exists as a non-covalent homodimer with an antiparallel conformation. Ca(2+) binding to S100A11 would trigger conformational changes which would expose the hydrophobic cleft of S100A11 and facilitate its interaction with target proteins. As a dual cell growth mediator, S100A11 acts as either a tumor suppressor or promoter in many different types of tumors and would play respective roles in influencing the proliferation of the cancer cells. In the nucleus, S100A11 suppresses the growth of keratinocytes through p21 (CIP1/WAF1) activation and induces cell differentiation. S100A11 is also a novel test marker in breast carcinoma.
References
Miyasaki KT. et al., 1993, J Dent Res. 72: 517-23.Ohuchida K. et al., 2006, Clin Cancer Res 12 (18): 5417-22.
Kouno T. et al., 2008, J Pept Sci. 14 (10): 1129-38.He H. et al., 2009, Cell Biochem Biophys 55 (3): 117-26.
Liu XG. et al., 2010, Oncol Rep. 23 (5): 1301-8.
Kouno T. et al., 2008, J Pept Sci. 14 (10): 1129-38.He H. et al., 2009, Cell Biochem Biophys 55 (3): 117-26.
Liu XG. et al., 2010, Oncol Rep. 23 (5): 1301-8.
Similar Products
Product Notes
The S100A11 (Catalog #AAA259126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-S100A11 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's S100A11 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the S100A11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.