WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells using S100A16 antibody.)
Rabbit S100A16 Polyclonal Antibody | anti-S100A16 antibody
S100A16 Antibody
Gene Names
S100A16; AAG13; S100F; DT1P1A7
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
S100A16, Antibody; S100A16 Antibody; 2300002L21Rik; AAG13; Aging associated gene 13 protein; Aging associated protein 13; Aging-associated gene 13 protein; AI325039; AI663996; DT1P1A7; MGC17528; Protein S100 A16; Protein S100-A16; Protein S100-F; Protein S100F; S100 calcium binding protein A16; S100 calcium-binding protein A16; S100A16; S100F; S10AG_HUMAN; anti-S100A16 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
S100A16 antibody detects endogenous levels of total S100A16
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
103
Applicable Applications for anti-S100A16 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus > Nucleolus. Cytoplasm. Primarily nucleolar. Translocates from the nucleolus to the cytoplasm in response to an increase of intracellular calcium levels.
Tissue Specificity
Ubiquitous (PubMed:14684152). Highly expressed in esophagus, adipose tissues and colon. Expressed at lower level in lung, brain, pancreas and skeletal muscle. Expression is up-regulated in tumors of bladder, lung, thyroid gland, pancreas and ovary (PubMed:14684152). Expressed in astrocytes (PubMed:17030513).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (90%), Bovine (90%), Horse (90%), Sheep (90%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells using S100A16 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells using S100A16 antibody.)
IHC (Immunohiostchemistry)
(AAA325008 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA325008 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA325008 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325008 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-S100A16 antibody
Function: Calcium-binding protein. Binds one calcium ion per monomer (PubMed:17030513). Can promote differentiation of adipocytes (in vitro) (By similarity). Overexpression in preadipocytes increases their proliferation, enhances adipogenesis and reduces insulin-stimulated glucose uptake (By similarity).
Subunit Structure: Homodimer (PubMed:17030513, PubMed:21046186). Interacts with TP53 (By similarity).
Similarity: S100A16 proteins, but not other S100 proteins, have only one functional Ca2+ binding site per monomer. Upon Ca2+ binding, undergoes conformational changes leading to the exposure of hydrophobic patches which could be implicated in the Ca2+-dependent nuclear export. Binds Zn2+. Ca2+ and Zn2+ do not bind to the same site. Does not bind Cu2+. Belongs to the S-100 family.
Subunit Structure: Homodimer (PubMed:17030513, PubMed:21046186). Interacts with TP53 (By similarity).
Similarity: S100A16 proteins, but not other S100 proteins, have only one functional Ca2+ binding site per monomer. Upon Ca2+ binding, undergoes conformational changes leading to the exposure of hydrophobic patches which could be implicated in the Ca2+-dependent nuclear export. Binds Zn2+. Ca2+ and Zn2+ do not bind to the same site. Does not bind Cu2+. Belongs to the S-100 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 20 kD
Predicted: 12 kDa
Predicted: 12 kDa
NCBI Official Full Name
protein S100-A16
NCBI Official Synonym Full Names
S100 calcium binding protein A16
NCBI Official Symbol
S100A16
NCBI Official Synonym Symbols
AAG13; S100F; DT1P1A7
NCBI Protein Information
protein S100-A16
UniProt Protein Name
Protein S100-A16
UniProt Gene Name
S100A16
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Product Notes
The S100A16 s100a16 (Catalog #AAA325008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S100A16 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100A16 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the S100A16 s100a16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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