IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)
Rabbit anti-Mouse, Rat S100A9 Polyclonal Antibody | anti-S100A9 antibody
Anti-S100A9 Antibody
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen Affinity Purified
Synonyms
S100A9, Antibody; Anti-S100A9 Antibody; Protein S100-A9; Leukocyte L1 complex heavy chain; 60B8AG; AW546964; BEE22; CAGB; Calgranulin B; Calgranulin-B; Calprotectin L1H subunit; CFAG; CGLB; Cystic fibrosis antigen; Cystic fibrosis antigen B; HGNC:10499; L1AG; LIAG; MAC 387; MAC387; MIF; Migration inhibitory factor related protein 14; Migration inhibitory factor-related protein 14; MRP 14; MRP 8; MRP-14; MRP14; Myeloid-related protein 14; NIF; OTTHUMP00000015331; P14; S100 A9; S100 calcium binding protein A9; S100 calcium binding protein A9 calgranulin B; S100 calcium-binding protein A9; S100A9; S10A9_HUMAN; anti-S100A9 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Applicable Applications for anti-S100A9 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-p), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes
ELISA:
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
IHC-p
Concentration: 0.5-1ug/ml
Tested Species: Mouse , Rat
Antigen Retrieval: By Heat
ICC/IF:
Concentration: 5ug/mL
Tested Species: Mouse, Rat
Flow Cytometry:
Concentration: 1-3ug/1x106 cells
Tested Species: Mouse
Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
IHC-p
Concentration: 0.5-1ug/ml
Tested Species: Mouse , Rat
Antigen Retrieval: By Heat
ICC/IF:
Concentration: 5ug/mL
Tested Species: Mouse, Rat
Flow Cytometry:
Concentration: 1-3ug/1x106 cells
Tested Species: Mouse
Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E Coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.
Cross Reactivity
Cross-reactivity with recombinant human S100A9 is observed.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detections Systems
The lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (Please inquire) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC(P) and ICC.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a 6 months.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)
IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)
IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Mouse Spleen Tissue)
IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Mouse Spleen Tissue)
WB (Western Blot)
(Anti- S100A9 Picoband antibody, AAA11645, Western blottingAll lanes: Anti S100A9 (AAA11645) at 0.5ug/mlWB: Mouse Ovary Tissue Lysate at 50ugPredicted bind size: 13KDObserved bind size: 13KD)
WB (Western Blot)
(Anti- S100A9 Picoband antibody, AAA11645, Western blottingAll lanes: Anti S100A9 (AAA11645) at 0.5ug/mlWB: Mouse Ovary Tissue Lysate at 50ugPredicted bind size: 13KDObserved bind size: 13KD)
Related Product Information for anti-S100A9 antibody
Background: S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.
References
1. "Entrez Gene: S100A9 S100 calcium binding protein A9". 2. Cheng P, Corzo CA, Luetteke N, Yu B, Nagaraj S, Bui MM, Ortiz M, Nacken W, Sorg C, Vogl T, Roth J, Gabrilovich DI (September 2008). "Inhibition of dendritic cell differentiation and accumulation of myeloid-derived suppressor cells in cancer is regulated by S100A9 protein". J. Exp. Med. 205 (10): 2235-49.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9 (calgranulin B)
NCBI Official Symbol
S100a9
NCBI Official Synonym Symbols
p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
NCBI Protein Information
protein S100-A9
UniProt Protein Name
Protein S100-A9
UniProt Gene Name
S100a9
UniProt Synonym Gene Names
Cagb; Mrp14; MRP-14; p14
UniProt Entry Name
S10A9_MOUSE
Similar Products
Product Notes
The S100A9 s100a9 (Catalog #AAA11645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-S100A9 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100A9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-p), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC). ELISA: Concentration: 0.1-0.5ug/ml Tested Species: Mouse Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Mouse IHC-p Concentration: 0.5-1ug/ml Tested Species: Mouse, Rat Antigen Retrieval: By Heat ICC/IF: Concentration: 5ug/mL Tested Species: Mouse, Rat Flow Cytometry: Concentration: 1-3ug/1x106 cells Tested Species: Mouse Tested Species: In-house tested species with positive results By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the S100A9 s100a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.