IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit S6K (RPS6KB1) Polyclonal Antibody | anti-RPS6KB1 antibody
S6K (RPS6KB1) Antibody (S424)
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
S6K (RPS6KB1), Antibody; S6K (RPS6KB1) Antibody (S424); Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; RPS6KB1; STK14A; anti-RPS6KB1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This S6K (RPS6KB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from human S6K (RPS6KB1).
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
425-454
Sequence Length
502
Applicable Applications for anti-RPS6KB1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (AAA284132) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (AAA284132))
WB (Western Blot)
(Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (AAA284132) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (AAA284132))
WB (Western Blot)
(Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) (RB11404). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).))
WB (Western Blot)
(Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) (RB11404). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).))
Related Product Information for anti-RPS6KB1 antibody
RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.
Product Categories/Family for anti-RPS6KB1 antibody
References
Adem, C., et al., Genes Chromosomes Cancer 41(1):1-11 (2004).
Suzuki, Y., et al., Genome Res. 14(9):1711-1718 (2004).
Gomez-Cambronero, J., et al., Leuk. Res. 28(7):755-762 (2004).
Raught, B., et al., EMBO J. 23(8):1761-1769 (2004).
Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).
Suzuki, Y., et al., Genome Res. 14(9):1711-1718 (2004).
Gomez-Cambronero, J., et al., Leuk. Res. 28(7):755-762 (2004).
Raught, B., et al., EMBO J. 23(8):1761-1769 (2004).
Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59140
NCBI Official Full Name
ribosomal protein S6 kinase beta-1 isoform e
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol
RPS6KB1
NCBI Official Synonym Symbols
S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
NCBI Protein Information
ribosomal protein S6 kinase beta-1
UniProt Protein Name
Ribosomal protein S6 kinase beta-1
UniProt Gene Name
RPS6KB1
UniProt Synonym Gene Names
STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
UniProt Entry Name
KS6B1_HUMAN
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Product Notes
The RPS6KB1 rps6kb1 (Catalog #AAA284132) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 425-454. The S6K (RPS6KB1) Antibody (S424) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's S6K (RPS6KB1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RPS6KB1 rps6kb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S6K (RPS6KB1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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