WB (Western Blot)
(Anti-RPS6KB1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human S6K1/RPS6KB1 Polyclonal Antibody | anti-RPS6KB1 antibody
Anti-S6K1/RPS6KB1 Antibody, Rabbit Polyclonal
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
S6K1/RPS6KB1, Antibody; Anti-S6K1/RPS6KB1 Antibody, Rabbit Polyclonal; PS6K/RPS6KB1 Antibody, Rabbit PAb, Antigen Affinity Purified; ribosomal protein S6 kinase, polypeptide 1; Anti-p70 S6KA Antibody; Anti-p70(S6K)-alpha Antibody; Anti-p70-alpha Antibody; Anti-p70-S6K Antibody; Anti-PS6K Antibody; Anti-RPS6KB1 Antibody; Anti-S6K Antibody; Anti-S6K-beta-1 Antibody; Anti-S6K1 Antibody; Anti-STK14A Antibody; anti-RPS6KB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PS6K/RPS6KB1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-RPS6KB1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Recombinant Human PS6K/RPS6KB1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human PS6K/RPS6KB1 (rh PS6K/RPS6KB1). PS6K/RPS6KB1 specific IgG was purified by Human PS6K/RPS6KB1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-RPS6KB1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-RPS6KB1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(RPS6KB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-RPS6KB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RPS6KB1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size: 59 kDa)
IP (Immunoprecipitation)
(RPS6KB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-RPS6KB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RPS6KB1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size: 59 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of RPS6KB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RPS6KB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of RPS6KB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RPS6KB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.)
Related Product Information for anti-RPS6KB1 antibody
PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K). S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR(rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1). Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.
Product Categories/Family for anti-RPS6KB1 antibody
References
Panasyuk, et al. (2006) Nuclear export of PS6K II is regulated by protein kinase CK2 phosphorylation at Ser-17. J Biol Chem. 281(42):31188-201.Carnevalli L, et al. (2010) PS6K Plays a Critical Role in Early Adipocyte Differentiation. Dev Cell. 18 (5):763-74.Grove JR, et al. (1991) Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 11(11):5541-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,140 Da
NCBI Official Full Name
ribosomal protein S6 kinase beta-1 isoform e
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol
RPS6KB1
NCBI Official Synonym Symbols
S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
NCBI Protein Information
ribosomal protein S6 kinase beta-1; p70 S6 kinase, alpha; ribosomal protein S6 kinase I; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A
UniProt Protein Name
Ribosomal protein S6 kinase beta-1
UniProt Gene Name
RPS6KB1
UniProt Synonym Gene Names
STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
UniProt Entry Name
KS6B1_HUMAN
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Product Notes
The RPS6KB1 rps6kb1 (Catalog #AAA254423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-S6K1/RPS6KB1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's S6K1/RPS6KB1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RPS6KB1 rps6kb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S6K1/RPS6KB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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