WB (Western Blot)
(Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody AAA211030 (lot AAA211030-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse S6K2 Polyclonal Antibody | anti-S6K2 antibody
Rabbit anti-S6K2 Antibody, Affinity Purified
Gene Names
RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
S6K2, Antibody; Rabbit anti-S6K2 Antibody, Affinity Purified; SRK; S6 kinase-related kinase; S6K2; S6KB; STK14B; S6Kbeta; S6K-beta; S6K-beta2; S6K-beta-2; S6KI(2); serine/threonine-protein kinase 14B; P70-beta; ribosomal protein S6 kinase, 70kDa, polypeptide 2; serine/threonine-protein kinase 14 beta; p70 ribosomal S6 kinase beta; ribosomal protein S6 kinase beta-2; P70-beta-2; KLS; p70 S6 kinase beta; p70 S6KB; p70 S6K-beta; p70(S6K)-beta; P70-beta-1; p70-S6K 2; P70S6K2; p70S6Kb; 70 kDa ribosomal protein S6 kinase 2; Ribosomal protein S6 kinase beta-2; ribosomal protein S6 kinase B2; anti-S6K2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-S6K2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 432 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody AAA211030 (lot AAA211030-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody AAA211030 (lot AAA211030-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody AAA211030 (lot AAA211030-2) used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody For blotting immunoprecipitated S6K2, AAA211030 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody AAA211030 (lot AAA211030-2) used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody For blotting immunoprecipitated S6K2, AAA211030 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-S6K2 antibody
Ribosomal protein S6 kinase beta 2 (S6K2) encodes a member of the ribosomal S6 kinase family that phosphorylates the 40 S ribosomal subunit protein S6 and eukaryotic translation initiation factor 4B (eIF4B). S6K2 is an isoform of the p70 S6 kinase, S6K (p70a/S6K1) and is in the PI3K and mTOR pathway.
Product Categories/Family for anti-S6K2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,455 Da
NCBI Official Full Name
ribosomal protein S6 kinase beta-2
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 70kDa, polypeptide 2
NCBI Official Symbol
RPS6KB2
NCBI Official Synonym Symbols
KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
NCBI Protein Information
ribosomal protein S6 kinase beta-2; P70S6K2; S6K-beta; p70 S6KB; p70-S6K 2; S6K-beta-2; p70 S6K-beta; p70 S6 kinase beta; S6 kinase-related kinase; p70 ribosomal S6 kinase beta; serine/threonine-protein kinase 14B; 70 kDa ribosomal protein S6 kinase 2; se
UniProt Protein Name
Ribosomal protein S6 kinase beta-2
UniProt Gene Name
RPS6KB2
UniProt Synonym Gene Names
STK14B; S6K-beta-2; S6K2; P70S6K2; p70-S6K 2; SRK; S6K-beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70-beta
UniProt Entry Name
KS6B2_HUMAN
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Product Notes
The S6K2 rps6kb2 (Catalog #AAA211030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-S6K2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's S6K2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the S6K2 rps6kb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S6K2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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