WB (Western Blot)
(Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody AAA210792 (lot AAA210792-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Goat anti-Human SA1 Polyclonal Antibody | anti-SA1 antibody
Goat anti-SA1 Antibody, Affinity Purified
Gene Names
STAG1; SA1; SCC3A
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SA1, Antibody; Goat anti-SA1 Antibody, Affinity Purified; MRD47; Stromal antigen 1; SCC3A; SCC3 homolog 1; nuclear protein stromal antigen 1; cohesin subunit SA-1; SA1; Cohesin subunit SA-1; stromal antigen 1; anti-SA1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SA1 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1150 and 1200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody AAA210792 (lot AAA210792-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody AAA210792 (lot AAA210792-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody AAA210792 (lot AAA210792-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210792-2). For blotting immunoprecipitated SA1, AAA210792 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody AAA210792 (lot AAA210792-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210792-2). For blotting immunoprecipitated SA1, AAA210792 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
ICC (Immunocytochemistry)
(Localization of human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; AAA210792) used at 1 ug/ml.)
ICC (Immunocytochemistry)
(Localization of human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; AAA210792) used at 1 ug/ml.)
Related Product Information for anti-SA1 antibody
SA1 is a component of the multisubunit cohesin complex. Cohesin functions to hold sister chromatids together during meiosis and mitosis and ensure proper segregation of chromosomes. SA1 has also been identified as a mediator of p53 induced apoptosis.
Product Categories/Family for anti-SA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140,090 Da
NCBI Official Full Name
cohesin subunit SA-1
NCBI Official Synonym Full Names
stromal antigen 1
NCBI Official Symbol
STAG1
NCBI Official Synonym Symbols
SA1; SCC3A
NCBI Protein Information
cohesin subunit SA-1; SCC3 homolog 1; nuclear protein stromal antigen 1
UniProt Protein Name
Cohesin subunit SA-1
UniProt Gene Name
STAG1
UniProt Synonym Gene Names
SA1
UniProt Entry Name
STAG1_HUMAN
Similar Products
Product Notes
The SA1 stag1 (Catalog #AAA210792) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SA1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SA1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SA1 stag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.