WB (Western Blot)
(Detection of human SA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SA2 antibody AAA211935 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
Rabbit anti-Human SA2 Polyclonal Antibody | anti-SA2 antibody
Rabbit anti-SA2 Antibody, Affinity Purified
Gene Names
STAG2; SA2; SA-2; SCC3B; bA517O1.1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SA2, Antibody; Rabbit anti-SA2 Antibody, Affinity Purified; bA517O1.1; SCC3 homolog 2; SCC3B; Stromal antigen 2; cohesin subunit SA-2; SA2; SA-2; Cohesin subunit SA-2; stromal antigen 2; anti-SA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SA2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SA2 antibody AAA211935 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of human SA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SA2 antibody AAA211935 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
IHC (Immunohistochemistry)
(Detection of human SA2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SA2 (Cat. No. AAA211935 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SA2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SA2 (Cat. No. AAA211935 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-SA2 antibody
SA2 is a component of the multisubunit cohesin complex. Cohesin functions to hold sister chromatids together during meiosis and mitosis and ensure proper segregation of chromosomes. Cohesin must dissociate from sister chromatids during prophase and metaphase so that they can segregate to opposite poles of the cell during anaphase. As part of the cohesin complex, SA2 appears to play a role in cohesin dissociation required for sister chromatid separation. Interestingly, SA2 has been demonstrated to also exhibit transcriptional regulatory activity through an association with NF-kappaB.
Product Categories/Family for anti-SA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
145,751 Da
NCBI Official Full Name
cohesin subunit SA-2 isoform b
NCBI Official Synonym Full Names
stromal antigen 2
NCBI Official Symbol
STAG2
NCBI Official Synonym Symbols
SA2; SA-2; SCC3B; bA517O1.1
NCBI Protein Information
cohesin subunit SA-2; SCC3 homolog 2
UniProt Protein Name
Cohesin subunit SA-2
UniProt Gene Name
STAG2
UniProt Synonym Gene Names
SA2
UniProt Entry Name
STAG2_HUMAN
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Product Notes
The SA2 stag2 (Catalog #AAA211935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SA2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SA2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SA2 stag2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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