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WB (Western Blot)
(AOS1 Antibody (V315) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).)
Rabbit SAE1 (AOS1) Polyclonal Antibody | anti-SAE1 antibody
SAE1 (AOS1) Antibody (C-term)
Gene Names
SAE1; AOS1; SUA1; UBLE1A; HSPC140
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SAE1 (AOS1), Antibody; SAE1 (AOS1) Antibody (C-term); SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; N-terminally processed; SAE1; AOS1; SUA1; UBLE1A; anti-SAE1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
300-329
Sequence Length
299
Applicable Applications for anti-SAE1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(AOS1 Antibody (V315) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).)
WB (Western Blot)
(AOS1 Antibody (V315) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(The AOS1 C-term Pab is used in Western blot to detect AOS1 in mouse heart tissue lysate.)
WB (Western Blot)
(The AOS1 C-term Pab is used in Western blot to detect AOS1 in mouse heart tissue lysate.)
Related Product Information for anti-SAE1 antibody
The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Product Categories/Family for anti-SAE1 antibody
References
Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999).
Gong, L., et al., FEBS Lett. 448(1):185-189 (1999).
Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
Gong, L., et al., FEBS Lett. 448(1):185-189 (1999).
Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38450
NCBI Official Full Name
SUMO-activating enzyme subunit 1 isoform b
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
NCBI Protein Information
SUMO-activating enzyme subunit 1
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Gene Name
SAE1
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A
UniProt Entry Name
SAE1_HUMAN
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Product Notes
The SAE1 sae1 (Catalog #AAA285732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 300-329. The SAE1 (AOS1) Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SAE1 (AOS1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SAE1 sae1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAE1 (AOS1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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