WB (Western Blot)
(Western blot analysis of SAP Antibody (Center E300) in Y79,A2058,CEM cell line lysates(35ug/lane). SAP (arrow) was detected using the purified Pab.)
Rabbit anti-Human SAP Polyclonal Antibody | anti-PSAP antibody
SAP Antibody (Center E300)
Gene Names
PSAP; GLBA; SAP1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SAP, Antibody; SAP Antibody (Center E300); Prosaposin; Proactivator polypeptide; Saposin-A; Protein A; Saposin-B-Val; Saposin-B; Cerebroside sulfate activator; CSAct; Dispersin; Sphingolipid activator protein 1; SAP-1; Sulfatide/GM1 activator; Saposin-C; A1 activator; Co-beta-glucosidase; Glucosylceramidase activator; Sphingolipid activator protein 2; SAP-2; Saposin-D; Component C; Protein C; PSAP; GLBA; SAP1; anti-PSAP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the Central region of human SAP.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
285-314
Sequence Length
527
Applicable Applications for anti-PSAP antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12). Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
Cellular Location
Lysosome
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of SAP Antibody (Center E300) in Y79,A2058,CEM cell line lysates(35ug/lane). SAP (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SAP Antibody (Center E300) in Y79,A2058,CEM cell line lysates(35ug/lane). SAP (arrow) was detected using the purified Pab.)
Related Product Information for anti-PSAP antibody
PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.
Product Categories/Family for anti-PSAP antibody
References
Gunia,S., Virchows Arch. 454 (5), 573-579 (2009)
Kuchar,L., Am. J. Med. Genet. A 149A (4), 613-621 (2009)
Kuchar,L., Am. J. Med. Genet. A 149A (4), 613-621 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58113
NCBI Official Full Name
prosaposin isoform b preproprotein
NCBI Official Synonym Full Names
prosaposin
NCBI Official Symbol
PSAP
NCBI Official Synonym Symbols
GLBA; SAP1
NCBI Protein Information
prosaposin
UniProt Protein Name
Prosaposin
UniProt Gene Name
PSAP
UniProt Synonym Gene Names
GLBA; SAP1; CSAct; SAP-1; SAP-2
UniProt Entry Name
SAP_HUMAN
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Product Notes
The PSAP psap (Catalog #AAA288319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 285-314. The SAP Antibody (Center E300) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAP can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PSAP psap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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