Rabbit SAPAP1 Polyclonal Antibody | anti-NPHS1 antibody

SAPAP1 Antibody

Cat. Num. AAA40224

• Gene Names: NPHS1; CNF; NPHN; nephrin
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: SAPAP1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SAPAP1, Antibody; SAPAP1 Antibody; SAPAP1; CNF; NPHN; nephrin; Nephrin; Renal glomerulus-specific cell adhesion receptor; nephrosis 1, congenital, Finnish type (nephrin; anti-NPHS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins.
• Purity/Purification: SAPAP1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1241

• Applicable Applications for anti-NPHS1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: SAPAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human SAPAP1.
• Buffer: SAPAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SAPAP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of SAPAP1 in Human Brain cells with SAPAP1 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of SAPAP1 in human brain with SAPAP1 antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of SAPAP1 in rat brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-NPHS1 antibody

SAPAP1 Antibody: SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM).

NCBI and Uniprot Product Information

• NCBI GI #: 4758822
• NCBI GeneID: 4868
• NCBI Accession #: NP_004737
• NCBI GenBank Nucleotide #: NM_004646.3
• UniProt Accession #: O60500; A6NDH2; C3RX61
• Molecular Weight: 130,546 Da
• NCBI Official Full Name: nephrin
• NCBI Official Synonym Full Names: nephrosis 1, congenital, Finnish type (nephrin)
• NCBI Official Symbol: NPHS1
• NCBI Official Synonym Symbols: CNF; NPHN; nephrin
• NCBI Protein Information: nephrin; renal glomerulus-specific cell adhesion receptor
• UniProt Protein Name: Nephrin
• UniProt Gene Name: NPHS1
• UniProt Synonym Gene Names: NPHN
• UniProt Entry Name: NPHN_HUMAN