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IF (Immunofluorescence)
(Immunofluorescence - anti-Sapphire Ab using NIH3T3 cells transduced with Sapphire-Rab5a; cells were fixed with methanol and anti-Sapphire at 1/100;)
Goat Sapphire Polyclonal Antibody
anti-Sapphire
Reactivity
Transfected cells, Green Fluorescent Protein
Applications
Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Epitope affinity purified
Synonyms
Sapphire, Antibody; anti-Sapphire; A green fluorescent protein antibody; anti-Sapphire antibody
Host
Goat
Reactivity
Transfected cells, Green Fluorescent Protein
Clonality
Polyclonal
Isotype
IgG
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
Purity/Purification
Epitope affinity purified
Form/Format
?Polyclonal antibody supplied as a 100ul (3mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3mg/ml (varies by lot)
Applicable Applications for anti-Sapphire antibody
IEM (Immunoelectron Microscopy), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
Conjugation
Unconjugated
Immunogen
Purified recombinant fluorescent protein produced in E Coli.
Preparation and Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
IF (Immunofluorescence)
(Immunofluorescence - anti-Sapphire Ab using NIH3T3 cells transduced with Sapphire-Rab5a; cells were fixed with methanol and anti-Sapphire at 1/100;)
IF (Immunofluorescence)
(Immunofluorescence - anti-Sapphire Ab using NIH3T3 cells transduced with Sapphire-Rab5a; cells were fixed with methanol and anti-Sapphire at 1/100;)
Application Data
(Anti-Sapphire Ab at 1/2,500 dilution; 293HEK cells transfected with Sapphire; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Application Data
(Anti-Sapphire Ab at 1/2,500 dilution; 293HEK cells transfected with Sapphire; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
IF (Immunofluorescence)
(Immunofluorescence -Anti-Sapphire Ab using NIH3T3 cells transduced with Sapphire-Rab5a; cells were fixed with methanol andAnti-Sapphire at 1/100;)
IF (Immunofluorescence)
(Immunofluorescence -Anti-Sapphire Ab using NIH3T3 cells transduced with Sapphire-Rab5a; cells were fixed with methanol andAnti-Sapphire at 1/100;)
Reactivity Data
Reactivity Data
Related Product Information for anti-Sapphire antibody
Goat polyclonal antibody to Sapphire. Sapphire is a basic (constitutively fluorescent) long stokes shift fluorescent protein engineered from green fluorescent protein (GFP) isolated from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with low acid sensitivity. Sapphire is a ~27kDa protein that is optimally excited at a 399nm and has a maximum of emission at 511nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Product Categories/Family for anti-Sapphire antibody
Similar Products
Product Notes
The Sapphire (Catalog #AAA63213) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The anti-Sapphire reacts with Transfected cells, Green Fluorescent Protein and may cross-react with other species as described in the data sheet. AAA Biotech's Sapphire can be used in a range of immunoassay formats including, but not limited to, IEM (Immunoelectron Microscopy), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Sapphire for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sapphire, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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