WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using Phospho-SAR1A(Thr139) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Rabbit SAR1A Polyclonal Antibody | anti-SAR1A antibody
Phospho-SAR1A (Thr139) Antibody
Gene Names
SAR1A; SAR1; Sara; SARA1; masra2
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (83%), Xenopus (83%)
Applications
ELISA, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
SAR1A, Antibody; Phospho-SAR1A (Thr139) Antibody; COPII associated small GTPase; COPII-associated small GTPase; GTP binding protein SAR1a; GTP-binding protein SAR1a; Masra 2; Masra2; SAR 1; SAR1 gene homolog A; SAR1 homolog A (S. cerevisiae); SAR1A; SAR1a gene homolog (S cerevisiae) 1; SAR1a gene homolog 1 (S cerevisiae); SAR1a gene homolog 1; SAR1A_HUMAN; SARA 1; Sara; SARA1; anti-SAR1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (83%), Xenopus (83%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (83%), Xenopus (83%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-SAR1A (Thr139) Antibody detects endogenous levels of SAR1A only when phosphorylated at Thr139.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SAR1A antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human SAR1A around the phosphorylation site of Thr139.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts with B3GAT1.
Similarity
Belongs to the small GTPase superfamily. SAR1 family.
Subcellular Location
Endoplasmic reticulum. Golgi apparatus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using Phospho-SAR1A(Thr139) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using Phospho-SAR1A(Thr139) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-SAR1A (Thr139) Antibody.Lane 1: RAW264.7 cells(LPS 4h treatment), blocked with antigen-specific peptides,Lane 2: RAW264.7 cells(LPS 4h treatment),Lane 3: HepG2 cells(LPS 4h treatment).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-SAR1A (Thr139) Antibody.Lane 1: RAW264.7 cells(LPS 4h treatment), blocked with antigen-specific peptides,Lane 2: RAW264.7 cells(LPS 4h treatment),Lane 3: HepG2 cells(LPS 4h treatment).)
Related Product Information for anti-SAR1A antibody
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
198
NCBI Official Full Name
GTP-binding protein SAR1a
NCBI Official Synonym Full Names
secretion associated, Ras related GTPase 1A
NCBI Official Symbol
SAR1A
NCBI Official Synonym Symbols
SAR1; Sara; SARA1; masra2
NCBI Protein Information
GTP-binding protein SAR1a; SAR1 homolog A; SAR1 gene homolog A; SAR1a gene homolog 1; COPII-associated small GTPase
UniProt Protein Name
GTP-binding protein SAR1a
UniProt Gene Name
SAR1A
UniProt Synonym Gene Names
SAR1; SARA; SARA1
UniProt Entry Name
SAR1A_HUMAN
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Product Notes
The SAR1A sar1a (Catalog #AAA329417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SAR1A (Thr139) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (83%), Xenopus (83%) and may cross-react with other species as described in the data sheet. AAA Biotech's SAR1A can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the SAR1A sar1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAR1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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