<
IP (Immunoprecipitation)
(SARNP was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SARNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SARNP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :34 kDa)
Rabbit anti-Human SARNP Polyclonal Antibody | anti-SARNP antibody
Anti-SARNP Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SARNP, Antibody; Anti-SARNP Antibody, Rabbit Polyclonal; SARNP Antibody, Rabbit PAb, Antigen Affinity Purified; SAP domain containing ribonucleoprotein; Anti-CIP29 Antibody; Anti-HCC1 Antibody; Anti-HSPC316 Antibody; Anti-THO1 Antibody; anti-SARNP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SARNP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SARNP antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human SARNP fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SARNP fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SARNP was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SARNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SARNP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :34 kDa)
IP (Immunoprecipitation)
(SARNP was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SARNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SARNP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :34 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of SARNP in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SARNP polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of SARNP in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SARNP polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining of human SARNP in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human SARNP in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human SARNP in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human SARNP in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-SARNP rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:34 kDa)
WB (Western Blot)
(Anti-SARNP rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:34 kDa)
Related Product Information for anti-SARNP antibody
SARNP (SAP Domain Containing Ribonucleoprotein) is a Protein Coding gene. The SARNP gene, located on 12q13.2, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, and frog. This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in the expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. SARNP is widely expressed in testis, endometrium, and other tissues. Diseases associated with SARNP include Subglottis Neoplasm and Supraglottis Neoplasm. The related pathways of SARNP are Cleavage of Growing Transcript in the Termination Region and Transport of Mature Transcript to Cytoplasm.
References
Schumann S, et al. (2016) Interactions between kshv orf57 and the novel human trex proteins, chtop and cip29. J Gen Virol 97 (8): 1904-1910.Hashii Y, et al. (2004) A novel partner gene cip29 containing a sap domain with mll identified in infantile myelomonocytic leukemia. Leukemia 18 (9): 1546-1548.Choong ML, et al. (2001) An integrated approach in the discovery and characterization of a novel nuclear protein over-expressed in liver and pancreatic tumors. FEBS Lett 496 (2-3): 109-116.
Similar Products
Product Notes
The SARNP (Catalog #AAA256336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SARNP Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SARNP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SARNP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARNP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.