IP (Immunoprecipitation)
(Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody AAA211823 used for IP at 3 ug/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human SASH1 Polyclonal Antibody | anti-SASH1 antibody
Rabbit anti-SASH1 Antibody, Affinity Purified
Gene Names
SASH1; SH3D6A; dJ323M4; dJ323M4.1
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
SASH1, Antibody; Rabbit anti-SASH1 Antibody, Affinity Purified; SH3D6A; dJ323M4.1; proline-glutamate repeat-containing protein; SAM and SH3 domain-containing protein 1; SAM and SH3 domain containing 1; anti-SASH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SASH1 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 750 and 800
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody AAA211823 used for IP at 3 ug/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody AAA211823 used for IP at 3 ug/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti-SASH1 (Cat. No. AAA211823 Lot1) used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
IHC (Immunohistochemistry)
(Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti-SASH1 (Cat. No. AAA211823 Lot1) used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
Related Product Information for anti-SASH1 antibody
SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.
Product Categories/Family for anti-SASH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136,653 Da
NCBI Official Full Name
SAM and SH3 domain-containing protein 1
NCBI Official Synonym Full Names
SAM and SH3 domain containing 1
NCBI Official Symbol
SASH1
NCBI Official Synonym Symbols
SH3D6A; dJ323M4; dJ323M4.1
NCBI Protein Information
SAM and SH3 domain-containing protein 1; 2500002E12Rik; proline-glutamate repeat-containing protein
UniProt Protein Name
SAM and SH3 domain-containing protein 1
UniProt Gene Name
SASH1
UniProt Synonym Gene Names
KIAA0790; PEPE1
UniProt Entry Name
SASH1_HUMAN
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Product Notes
The SASH1 sash1 (Catalog #AAA211823) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SASH1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SASH1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the SASH1 sash1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SASH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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