Rabbit anti-Human Scavenging Receptor SRB2/SCARB2 Polyclonal Antibody | anti-SCARB2 antibody

Anti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband

Cat. Num. AAA19535

• Gene Names: SCARB2; AMRF; EPM4; LGP85; CD36L2; HLGP85; LIMP-2; LIMPII; SR-BII
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Scavenging Receptor SRB2/SCARB2, Antibody; Anti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband; anti-SCARB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-SCARB2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 0.25-0.5 ug/ml, Human; IHC-P: 2-5 ug/ml, Human; FC/FACS: 1-3 ug/1x10^6 cells, Human; Direct ELISA: 0.1-0.5 ug/ml, Human; Tested Species: In-house tested species with positive results.; Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human Scavenging Receptor SRB2/SCARB2 recombinant protein (Position: E48-H357).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 7. Flow Cytometry analysis of U87 cells using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Overlay histogram showing U87 cells stained with AAA19535 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human SH-SY5Y whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 antigen affinity purified polyclonal antibody (#AAA19535) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Scavenging Receptor SRB2/SCARB2 at approximately 80 kDa. The expected band size for Scavenging Receptor SRB2/SCARB2 is at 54 kDa.)

Related Product Information for anti-SCARB2 antibody

Lysosomal integral membrane protein 2 (LIMP-2) is a protein that in humans is encoded by the SCARB2 gene. The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is a ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-SCARB2 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 2498525
• NCBI GeneID: 950
• UniProt Accession #: Q14108; Q53Y63; B4DKD8; E7EM68
• Molecular Weight: 54,290 Da
• NCBI Official Full Name: Lysosome membrane protein 2
• NCBI Official Synonym Full Names: scavenger receptor class B, member 2
• NCBI Official Symbol: SCARB2
• NCBI Official Synonym Symbols: AMRF; EPM4; LGP85; CD36L2; HLGP85; LIMP-2; LIMPII; SR-BII
• NCBI Protein Information: lysosome membrane protein 2; LIMP II; CD36 antigen-like 2; lysosome membrane protein II; 85 kDa lysosomal membrane sialoglycoprotein; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; CD36 antigen (collagen type I receptor, thrombos
• UniProt Protein Name: Lysosome membrane protein 2
• UniProt Gene Name: SCARB2
• UniProt Synonym Gene Names: CD36L2; LIMP2; LIMPII; LGP85; LIMP II
• UniProt Entry Name: SCRB2_HUMAN