WB (Western Blot)
(Western blot analysis of Scn1a expression in rat brain extract (lane 1), mouse brain extract (lane 2) and U87 whole cell lysates (lane 4). Scn1a at 250KD was detected using rabbit anti- Scn1a Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Scn1a Polyclonal Antibody | anti-SCN1A antibody
Anti-Scn1a Antibody
Gene Names
SCN1A; FEB3; FHM3; NAC1; SCN1; SMEI; EIEE6; FEB3A; HBSCI; GEFSP2; Nav1.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Scn1a, Antibody; Anti-Scn1a Antibody; Sodium channel protein type 1 subunit alpha; brain sodium channel type I; EIEE6; FEB3; FEB3A; FHM3; GEFS+2; GEFSP2; HBSC I; HBSCI; NAC1; Nav 1.1; RBI; SCN1; Scn1a; SCN1A_HUMAN; SMEI; Sodium channel protein brain I alpha subunit; Sodium channel protein brain I subunit alpha; Sodium channel protein type I subunit alpha; Sodium channel voltage gated type 1 alpha subunit; Sodium channel voltage gated type I alpha polypeptide; Voltage-gated sodium channel subunit alpha Nav1.1; sodium voltage-gated channel alpha subunit 1; anti-SCN1A antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
2009
Applicable Applications for anti-SCN1A antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Scn1a (1981-2009aa ACPPSYDRVTKPIVEKHEQEGKDEKAKGK), identical to the related rat sequence.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Western blot analysis of Scn1a expression in rat brain extract (lane 1), mouse brain extract (lane 2) and U87 whole cell lysates (lane 4). Scn1a at 250KD was detected using rabbit anti- Scn1a Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Scn1a expression in rat brain extract (lane 1), mouse brain extract (lane 2) and U87 whole cell lysates (lane 4). Scn1a at 250KD was detected using rabbit anti- Scn1a Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-SCN1A antibody
Description: Rabbit IgG polyclonal antibody for Sodium channel protein type 1 subunit alpha(SCN1A) detection. Tested with WB in Human;Mouse;Rat.
Background: Nav1.1, also known as the sodium channel, voltage-gated, type I, alpha subunit (SCN1A), is a protein which in humans is encoded by the SCN1A gene. Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript.
Background: Nav1.1, also known as the sodium channel, voltage-gated, type I, alpha subunit (SCN1A), is a protein which in humans is encoded by the SCN1A gene. Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript.
References
1. "Entrez Gene: SCN1A sodium channel, voltage-gated, type I, alpha subunit". 2. Ito M, Nagafuji H, Okazawa H, Yamakawa K, Sugawara T, Mazaki-Miyazaki E, Hirose S, Fukuma G, Mitsudome A, Wada K, Kaneko S (Jan 2002)."Autosomal dominant epilepsy with febrile seizures plus with missense mutations of the (Na+)-channel alpha 1 subunit gene, SCN1A". Epilepsy Research 48 (1-2): 15-23. 3. Malo MS, Blanchard BJ, Andresen JM, Srivastava K, Chen XN, Li X, Jabs EW, Korenberg JR, Ingram VM (1994). "Localization of a putative human brain sodium channel gene (SCN1A) to chromosome band 2q24". Cytogenetics and Cell Genetics 67 (3): 178-86.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
226,174 Da
NCBI Official Full Name
sodium channel protein type 1 subunit alpha isoform 1
NCBI Official Synonym Full Names
sodium voltage-gated channel alpha subunit 1
NCBI Official Symbol
SCN1A
NCBI Official Synonym Symbols
FEB3; FHM3; NAC1; SCN1; SMEI; EIEE6; FEB3A; HBSCI; GEFSP2; Nav1.1
NCBI Protein Information
sodium channel protein type 1 subunit alpha
UniProt Protein Name
Sodium channel protein type 1 subunit alpha
UniProt Gene Name
SCN1A
UniProt Synonym Gene Names
NAC1; SCN1
UniProt Entry Name
SCN1A_HUMAN
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Product Notes
The SCN1A scn1a (Catalog #AAA46428) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Scn1a Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Scn1a can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SCN1A scn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Scn1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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