Application Data
(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Rabbit SDF-1 ALPHA Polyclonal Antibody | anti-SDF-1 ALPHA antibody
RABBIT ANTI HUMAN SDF-1 ALPHA:Biotin
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Applications
Western Blot, ELISA
Synonyms
SDF-1 ALPHA, Antibody; RABBIT ANTI HUMAN SDF-1 ALPHA:Biotin; anti-SDF-1 ALPHA antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Purified IgG conjugated to Biotin - lyophilised
Concentration
IgG concentration 0.1 mg/ml after reconstitution (varies by lot)
Sequence Length
93
Applicable Applications for anti-SDF-1 ALPHA antibody
WB (Western Blot), ELISA
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antisera to human SDF-1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Related Product Information for anti-SDF-1 ALPHA antibody
Rabbit anti Human SDF-1 alpha antibody recognizes human SDF-1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform. SDF-1 binds with high-affinity to the G protein -coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,004 Da
NCBI Official Full Name
stromal cell-derived factor 1 isoform beta
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
CXCL12
NCBI Official Synonym Symbols
IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
NCBI Protein Information
stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Gene Name
CXCL12
UniProt Synonym Gene Names
SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
UniProt Entry Name
SDF1_HUMAN
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Product Notes
The SDF-1 ALPHA cxcl12 (Catalog #AAA49833) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SDF-1 ALPHA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SDF-1 ALPHA cxcl12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDF-1 ALPHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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