WB (Western Blot)
(Detection of human and mouse Sec62 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody AAA212425 (lot AAA212425-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse Sec62 Polyclonal Antibody | anti-Sec62 antibody
Rabbit anti-Sec62 Antibody, Affinity Purified
Gene Names
SEC62; HTP1; TP-1; Dtrp1; TLOC1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Sec62, Antibody; Rabbit anti-Sec62 Antibody, Affinity Purified; hTP-1; translocation protein SEC62; translocation protein 1; TP-1; TLOC1; membrane protein SEC62, S.cerevisiae, homolog of; HTP1; Dtrp1; SEC62 preprotein translocation factor; Translocation protein SEC62; SEC62 homolog, preprotein translocation factor; anti-Sec62 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Sec62 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 349 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Sec62 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody AAA212425 (lot AAA212425-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse Sec62 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody AAA212425 (lot AAA212425-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Sec62 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody AAA212425 (lot AAA212425-1) used for IP at 6 ug/mg lysate. Sec62 was also immunoprecipitated by rabbit anti-Sec62 antibody BL14372. For blotting immunoprecipitated Sec62, AAA212425 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Sec62 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody AAA212425 (lot AAA212425-1) used for IP at 6 ug/mg lysate. Sec62 was also immunoprecipitated by rabbit anti-Sec62 antibody BL14372. For blotting immunoprecipitated Sec62, AAA212425 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-Sec62 antibody
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Sec62 and Sec63 protein are found to be associated with ribosome-free Sec61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Sec62 is an integral membrane protein located in the rough ER [taken from NCBI Entrez Gene (Gene ID: 7095)].
Product Categories/Family for anti-Sec62 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,862 Da
NCBI Official Full Name
translocation protein SEC62
NCBI Official Synonym Full Names
SEC62 homolog (S. cerevisiae)
NCBI Official Symbol
SEC62
NCBI Official Synonym Symbols
HTP1; TP-1; Dtrp1; TLOC1
NCBI Protein Information
translocation protein SEC62; hTP-1; translocation protein 1; membrane protein SEC62, S.cerevisiae, homolog of
UniProt Protein Name
Translocation protein SEC62
UniProt Gene Name
SEC62
UniProt Synonym Gene Names
TLOC1; TP-1; hTP-1
UniProt Entry Name
SEC62_HUMAN
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Product Notes
The Sec62 sec62 (Catalog #AAA212425) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Sec62 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sec62 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Sec62 sec62 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sec62, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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