WB (Western Blot)
(Detection of human Selenoprotein S by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody AAA212646 (lot AAA212646-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Selenoprotein S Polyclonal Antibody | anti-SELENOS antibody
Rabbit anti-Selenoprotein S Antibody, Affinity Purified
Gene Names
VIMP; SELS; ADO15; SBBI8; SEPS1; AD-015
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Selenoprotein S, Antibody; Rabbit anti-Selenoprotein S Antibody, Affinity Purified; tanis; VCP-interacting membrane protein; VIMP; VCP interacting membrane selenoprotein; valosin-containing protein-interacting membrane protein; SEPS1; SELS; selenoprotein S; SBBI8; AD-015; ADO15; Selenoprotein S; anti-SELENOS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SELENOS antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 139 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Selenoprotein S by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody AAA212646 (lot AAA212646-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human Selenoprotein S by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody AAA212646 (lot AAA212646-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Selenoprotein S by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody AAA212646 (lot AAA212646-1) used for IP at 6 ug per reaction. Selenoprotein S was also immunoprecipitated by rabbit anti-Selenoprotein S antibody BL16285. For blotting immunoprecipitated Selenoprotein S, AAA212646 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Selenoprotein S by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody AAA212646 (lot AAA212646-1) used for IP at 6 ug per reaction. Selenoprotein S was also immunoprecipitated by rabbit anti-Selenoprotein S antibody BL16285. For blotting immunoprecipitated Selenoprotein S, AAA212646 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SELENOS antibody
Selenoprotein S (SELS) is involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Selenoprotein S participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. It probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BQE4].
Product Categories/Family for anti-SELENOS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,163 Da
NCBI Official Full Name
selenoprotein S isoform 2
NCBI Official Synonym Full Names
VCP interacting membrane selenoprotein
NCBI Official Symbol
VIMP
NCBI Official Synonym Symbols
SELS; ADO15; SBBI8; SEPS1; AD-015
NCBI Protein Information
selenoprotein S
UniProt Protein Name
Selenoprotein S
UniProt Gene Name
VIMP
UniProt Synonym Gene Names
SELS; SelS
UniProt Entry Name
SELS_HUMAN
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Product Notes
The SELENOS vimp (Catalog #AAA212646) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Selenoprotein S Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Selenoprotein S can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SELENOS vimp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Selenoprotein S, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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