IHC (Immunohistochemisry)
(IHC image of AAA234128 diluted at 1:300 and staining in paraffin-embeddedhuman lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.)
Rabbit SEMA3A Polyclonal Antibody | anti-SEMA3A antibody
SEMA3A Antibody
Gene Names
SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
Reactivity
Human, Rat, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
SEMA3A, Antibody; SEMA3A Antibody; Semaphorin-3A; Semaphorin III; Sema III; SEMA3A; SEMAD; anti-SEMA3A antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
771
Applicable Applications for anti-SEMA3A antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human Semaphorin-3A protein (357-445AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(IHC image of AAA234128 diluted at 1:300 and staining in paraffin-embeddedhuman lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.)
IHC (Immunohistochemisry)
(IHC image of AAA234128 diluted at 1:300 and staining in paraffin-embeddedhuman lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.)
IHC (Immunohiostchemistry)
(IHC image of AAA234128 diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of AAA234128 diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Rat kidney tissue, Mouse heart tissue, Mouse skeletal muscleAll lanes: SEMA3 antibody at 3.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89 KDaObserved band size: 89 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Rat kidney tissue, Mouse heart tissue, Mouse skeletal muscleAll lanes: SEMA3 antibody at 3.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89 KDaObserved band size: 89 KDa)
Related Product Information for anti-SEMA3A antibody
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,889 Da
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
semaphorin 3A
NCBI Official Symbol
SEMA3A
NCBI Official Synonym Symbols
HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
NCBI Protein Information
semaphorin-3A
UniProt Protein Name
Semaphorin-3A
UniProt Gene Name
SEMA3A
UniProt Synonym Gene Names
SEMAD; Sema III
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Product Notes
The SEMA3A sema3a (Catalog #AAA234128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEMA3A Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA3A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SEMA3A sema3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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