IHC (Immunohistochemisry)
(Immunofluorescence staining of HepG2 cells with AAA114308 at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human SEMA5A Polyclonal Antibody | anti-SEMA5A antibody
SEMA5A Antibody
Gene Names
SEMA5A; semF; SEMAF
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA
Purity
>95%,Protein G purified
Synonyms
SEMA5A, Antibody; SEMA5A Antibody; Semaphorin-5A; Semaphorin-F; Sema F; SEMA5A; SEMAF; anti-SEMA5A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid; Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Sequence Length
1074
Applicable Applications for anti-SEMA5A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant human Semaphorin-5A protein (201-400AA)
Immunogen Species
Homo sapiens (Human)
Conjugation
Non-conjugated
Research Area
Others
Target Names
SEMA5A
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescence staining of HepG2 cells with AAA114308 at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohistochemisry)
(Immunofluorescence staining of HepG2 cells with AAA114308 at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using AAA114308 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using AAA114308 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung tissue using AAA114308 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung tissue using AAA114308 at dilution of 1:100)
Related Product Information for anti-SEMA5A antibody
Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
semaphorin-5A
NCBI Official Synonym Full Names
semaphorin 5A
NCBI Official Symbol
SEMA5A
NCBI Official Synonym Symbols
semF; SEMAF
NCBI Protein Information
semaphorin-5A
UniProt Protein Name
Semaphorin-5A
UniProt Gene Name
SEMA5A
UniProt Synonym Gene Names
SEMAF; Sema F
UniProt Entry Name
SEM5A_HUMAN
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Product Notes
The SEMA5A sema5a (Catalog #AAA114308) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEMA5A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA5A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SEMA5A sema5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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