Rabbit SEMA7A Polyclonal Antibody | anti-SEMA7A antibody

SEMA7A Antibody

Cat. Num. AAA325106

• Gene Names: SEMA7A; JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• Reactivity: Human,Mouse,Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Peptide affinity purification
• Synonyms: SEMA7A, Antibody; SEMA7A Antibody; CD 108; CD108; CD108 antigen; CDw108; H Sema K1; H Sema L; H-SEMA-K1; H-Sema-L; JMH; JMH blood group antigen; John Milton Hagen blood group H Sema K1; John Milton Hargen human blood group Ag; John-Milton-Hargen human blood group Ag; MGC126692; MGC126696; SEM7A_HUMAN; Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A; Sema K1; Sema L; SEMA7A; SEMAK1; SEMAL; Semaphorin 7A GPI membrane anchor; Semaphorin K1; Semaphorin L; Semaphorin-7A; Semaphorin-K1; Semaphorin-L; Semaphorin7a; anti-SEMA7A antibody

• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Clonality: Polyclonal
• Specificity: SEMA7A Antibody detects endogenous levels of total SEMA7A.
• Purity/Purification: Peptide affinity purification
• Form/Format: IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/m (varies by lot)

• Applicable Applications for anti-SEMA7A antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human SEMA7A, corresponding to a region within the internal amino acids.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts from CoLo cells using SEMA7A antibody.The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot)

(Western blot analysis of SEMA7A expression in Rat brain lysate)

IF (Immunofluorescence)

(AAA325106 staining CoLo by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-SEMA7A antibody

Function: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
Subunit Structure: Interacts with ITGA1 and ITGB1 (Probable). Interacts with PLXNC1.
Similarity: Belongs to the semaphorin family.

NCBI and Uniprot Product Information

• NCBI GI #: 225637520
• NCBI GeneID: 8482
• NCBI Accession #: NP_001139501.1
• NCBI GenBank Nucleotide #: NM_001146029.2
• UniProt Accession #: O75326; Q1XE81; Q1XE82; Q1XE83; Q1XE84; Q3MIY5; B4DDP7; F5H1S0
• Molecular Weight: 75KD
• NCBI Official Full Name: semaphorin-7A isoform 2
• NCBI Official Synonym Full Names: semaphorin 7A (John Milton Hagen blood group)
• NCBI Official Symbol: SEMA7A
• NCBI Official Synonym Symbols: JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• NCBI Protein Information: semaphorin-7A
• UniProt Protein Name: Semaphorin-7A
• UniProt Gene Name: SEMA7A
• UniProt Synonym Gene Names: CD108; SEMAL; Sema K1; Sema L