Rabbit Semaphorin-6A Polyclonal Antibody | anti-SEMA6A antibody

Semaphorin-6A (C-terminus)

Cat. Num. AAA71553

• Gene Names: SEMA6A; VIA; SEMA; HT018; SEMAQ; SEMA6A1
• Reactivity: Human, Rat, Mouse, Chicken
• Applications: Immunoprecipitation, ELISA, Immunocytochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: Semaphorin-6A, Antibody; Semaphorin-6A (C-terminus); Semaphorin VIA; Sema VIA; SEMA6A-1; KIAA1368; SEMAQ; anti-SEMA6A antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using Semaphorin 6A (C-terminus) peptide (without carrier). The antibody detects a 120kDa* protein corresponding to the apparent molecular mass of Sema-6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (C-terminus) antibody.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 1030

• Applicable Applications for anti-SEMA6A antibody: IP (Immunoprecipitation), ELISA, ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the C-terminus of human Sema-6A. The sequence used is identical to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of Sema-6A in COS7 cells that were mock transfected (top images) or Sema-6A transfected (bottom images). The cells were labeled with anti-Sema-6A (a.a. 772-787) (Left top and bottom image) or anti-Sema-6A (C-terminus) (Right top and bottom image). The antibodies were detected using anti-rabbit fluorescent secondary antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, University of Torino, Italy).)

WB (Western Blot)

(Western blot of Sema-6A in human colorectal cancer (CRC) cells treated with control (shC) or Sema-6A (shSema6A) shRNAs. Sema-6A was immunoprecipitated from each of the CRC lysates using Sema-6A (a.a. 772-787) or Sema-6A (C-terminus) antibody, then the blotted immunoprecipitations were probed with Sema-6A antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, Univ. of Torino, Italy).)

Related Product Information for anti-SEMA6A antibody

The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 6A (Sema-6A) is a single-pass type I membrane protein that contains a plexin-semaphorin-integrin (PSI) domain and a Sema domain. Sema-6A is active as a homodimer or oligomer. The Sema-6A homodimer interacts with a Plexin A2 homodimer. Sema-6a promotes reorganization of the actin cytoskeleton to regulate normal granule cell migration in the developing cerebellum and to control axon guidance in the developing central nervous system. In addition, Sema-6a has been implicated in oligodendrocyte myelination and may alter VEGF signaling during angiogenesis.

References

Xu, X.M. (2000) J Neurosci. 20(7):2638.Bernard, F. et al. (2012) Glia. 60(10):1590. Segarra, M. et al. (2012) Blood. 120(19):4104.

NCBI and Uniprot Product Information

• NCBI GI #: 11991660
• NCBI GeneID: 57556
• NCBI Accession #: NP_065847.1
• NCBI GenBank Nucleotide #: NM_020796.4
• UniProt Accession #: Q9H2E6
• Molecular Weight: 120 kDa
• NCBI Official Full Name: semaphorin-6A isoform 2
• NCBI Official Synonym Full Names: semaphorin 6A
• NCBI Official Symbol: SEMA6A
• NCBI Official Synonym Symbols: VIA; SEMA; HT018; SEMAQ; SEMA6A1
• NCBI Protein Information: semaphorin-6A
• UniProt Protein Name: Semaphorin-6A
• UniProt Gene Name: SEMA6A
• UniProt Synonym Gene Names: KIAA1368; SEMAQ; Sema VIA; SEMA6A-1